摘要
目的通过对胃癌患者术前镜检标本VEGF-C、CCR7蛋白表达的检测,探讨镜检标本VEGF-C、CCR7蛋白表达预测淋巴结转移的价值。方法采用Western blotting技术对37例胃癌镜检标本的VEGF-C、CCR7蛋白表达进行检测。结果 37例胃癌患者术前镜检标本中,VEGF-C蛋白表达的阳性率为54.1%(20/37);CCR7蛋白表达的阳性率为67.6%(25/37),通过ROC分析,联合检测镜检标本VEGF-C、CCR7表达对术前预测胃癌淋巴结转移有较高的准确性,阳性预测值为94.7%。结论对术前镜检标本采用Western blotting技术联合检测VEGF-C、CCR7蛋白表达对术前预测胃癌淋巴结转移具有一定的临床价值。
Objective To measure the combined examination of VEGF-C and CCR7 protein expression in endoscopic biopsy specimens of gastric carcinoma and assessed their usefulness as a diagnostic tools of predicting lymph node metastasis before surgery.Methods Endoscopic biopsy specimens（tumor and corresponding normal mucosa） of gastric tissue from 37 patients with gastric carcinoma who later underwent surgical resection,which were included in the current study.VEGF-C and CCR7 protein expression were examined in 37 cases of endoscopic biopsy specimens by Western-blotting assay.Results In the endoscopic biopsy specimens of 37 patients with gastric carcinoma,the positive rate of VEGF-C and CCR7 were 54.1%（20/37） and 67.6%（25/37）.By receiver operating characteristic（ROC） analysis,it showed that the accuracy of the combined examination of VEGF-C and CCR7 expression of endoscopic biopsy specimens in predicting lymph node metastasis before surgery was relatively high（area under ROC curve[Az=0.764].The positive predictive value（PPV） was 94.7%.Conclusion The accuracy of the combined examination of VEGF-C and CCR7 protein expression in predicting lymph node metastasis was relatively high.
出处
《中国实验诊断学》
北大核心
2010年第7期1049-1050,共2页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅自然科学发展基金资助项目(20010536)
