电阻抗法测量红细胞的流变特性

MEASUREMENT OF RBC RHEOLOGICAL BEHAVIOURS WITH IMPEDANCE METHOD

本文在ＦＲＩＣＫＥ悬浮液电导理论基础上，利用北医设计研制的红细胞流变特性测定仪（电阻抗法）测量红细胞的松弛过程。通过大量的实验证明，该仪器所测值确实能够反映出红细胞的流变特性，其稳定性和重复性也较好。变异系数均小于５％，并且与激光法测量值有较好的相关性。该测量方法虽受红细胞压积和悬浮介质电导率的影响和限制，但只要在仪器测量范围内，严格控制压积的一致性。

On the basis of Frich Velick's theory on conductivity of red blood cell (RBC) suspension, we studied the relaxation process of RBC with an erythrocyte rheometer (impedance method) developed by Beijing Medical University. It was shown from the results that this kind of method can really reflect the rheological properties of RBC with good stability and reproducibility. The coefficient of variation CV was lower than 5%, and comparing with that of ektacytometry, we found that there was a good correlation between the measuring results of the two methods. Although, this method was sometimes affected by the hematocrit of RBC and the conductivity of suspending medium, the reproducibility and accuracy of the results could be ensured. provided it was within the range of the instrument and the hematocrit of blood sample was strictly kept constant.