人血浆中辅酶Q_(10)的HPLC测定法及其动态研究

DETERMINATION OF COENZYME Q 10 AND ITS CONCENTRATION TIME CURVE IN HUMAN PLASMA BY HPLC

目的：建立人血浆中辅酶Ｑ１０的高效液相色谱检测法，以测定人体内辅酶Ｑ１０的经时变化过程。方法：血浆经无水乙醇沉淀蛋白后，以正己烷提取，进行高效液相色谱法检测。色谱柱为ＳｐｈｅｒｉｓｏｒｂＣ１８１０μｍ２５ｃｍ×４６ｍｍＩＤ，流动相为无水乙醇—水—冰醋酸（９８∶２∶０７），检测波长为２７５ｎｍ，内标为辅酶Ｑ９。结果：在０２～４０μｇ·ｍｌ－１浓度范围内峰面积比与浓度呈良好的线性关系，γ＝０９９９８，方法重现性好，提取回收率大于９０％；以本法测定了８名男性健康受试者服用辅酶Ｑ１０制剂前后血浆中辅酶Ｑ１０的浓度经时变化过程。结论：用本法测定人血浆中辅酶Ｑ１０浓度结果满意；人血浆中内源性辅酶Ｑ１０浓度为（７６３３±８６３）ｎｇ·ｍｌ－１。

AIM: To develope an HPLC method for the study of plasma concentration time curve of coenzyme Q 10 (CoQ 10 ) in human body. METHODS: Chromatography was performed on a Spherisorb C 18 column (25 cm×4 6 mm ID) with ethanol water acetic acid (98∶2∶0 7) as mobile phase. The detection wavelength was 275 nm. The internal standard was coenzyme Q 9 (CoQ 9 ). After deproteinization with ethanol, the plasma was extracted with n hexane. RESULTS: A good linearity was obtained from 0 2～4 0 μg·ml -1 of CoQ 10 in human plasma with a correlation coefficient of 0 9998. The extraction recovery was more than 90%. The plasma concentration time curve of CoQ 10 of eight volunteers was determined by this method following a controlled clinical experiment. CONCLUSION: The established HPLC method was proved to be a good mehtod for the determination of CoQ 10 in human plasma. The experimental results showed that the human plasma concentration of endogenous CoQ 10 was (763 3±86 3) ng·ml -1 and was affected by food and movement.