高效离子排斥色谱法测定蔬菜中的马来酰肼

Determination of maleic hydrazide in vegetables by high performance ion-exclusion chromatography

建立了马铃薯、洋葱、大蒜中马来酰肼(MH)的高效离子排斥色谱(HPIEC)检测方法。样品经提取、净化、过滤后进行色谱分析,以3mmol/L甲酸水溶液-乙腈(70:30,v/v)为流动相,流速0.8mL/min,采用IonpacICE-AS1(250mm×9mm)HPIEC色谱柱分离,紫外检测波长205nm,外标法定量。结果表明,MH的线性范围为0.006～1.0mg/L,相关系数为0.9999,回收率为91%～103%,相对标准偏差(RSD)均小于3%,检出限为0.002mg/L(S/N=3)。该方法灵敏度高,前处理简便,可用于马铃薯、洋葱、大蒜中MH的检测。

A new method was developed for the determination of maleic hydrazide （MH） in potatoes,onions and garlics by high performance ion-exclusion chromatography （HPIEC）.The sample was extracted with acidic methanol,and then analyzed by HPIEC.The analytical column was IonPac ICE-AS1 （250 mm×9 mm） and a mixture of 3 mmol/L formic acid water solution-acetonitrile （70：30,v/v） was used as the eluent at a flow rate of 0.8 mL/min.The detection was performed at 205 nm by an Ultimate 3000 VWD.The quantitative analysis of external standard calibration curves was used.The linear range of the method for MH was 0.006-1.0 mg/L （r=0.9999）.The average recoveries were 91%-103% with relative standard deviations （RSDs） less than 3%.The detection limit was 0.002 mg/L for MH.The method is simple,effective,precise,sensitive,reproducible and selective.It can be used to determine the residue of MH in potatoes,onions and garlics.