不同培养条件对牛膝发根诱导的研究

Effect of Different Conditions of Culture on Inducement of Hairy Roots of Achyranthes Bidentata Bl

目的:利用发根农杆菌A4,R1,1601,1025诱导牛膝,得到发根。方法:采用共培养法诱导牛膝发根,研究不同菌种类型、不同外植体、不同浸染时间、乙酰丁香酮浓度等条件对转化率的影响。结果:利用发根农杆菌1025浸染牛膝的叶片为转化材料,当感染8min,转化率最高。在培养基中添加50μmol.L-1乙酰丁香酮可以提高牛膝发根的转化效率。结论:用共培养法诱导出牛膝发根,并确定了最佳诱导条件,以提高牛膝外植体的诱导率。

Objective： To induce hairy roots of Achyranthes bidentata B1. by Agrobacterium rhizogenes A4, R1, 1601, 1025. Methods： Hairy roots were induced by co-culture. Effects of different types of bacteria, different explants, different infection time and different concentrations of acetosyringone on the transformation frequency were studied. Results： The highest induction frequency was obtained from leaves with Agrobacterium rhizogenes 1025 infection which were infected 8min. The transformation frequency could be raised by culture medium with 50μmol· L^ -1 acetosyringone. Conclusion： Hairy roots were induced by co-culture and the optimum induced condition was determined to improve the induction rate of explants.