几株纤维素酶产生菌的分离鉴定及其产酶能力

Isolation and Identification of Several Cellulase-producing Strains and Their Cellulase Productivity Ability

从枯枝、秸秆、土壤中分离出35株产纤维素酶菌株。以酶活值作为复筛标准,筛选出5株产羧甲基纤维素酶和滤纸酶活力相对较高的菌株,分别为柱隔孢霉(Ramularia NS1)、康氏木霉(Trichoderma koningii NS2)、灰绿青霉(Penicillium glaucum NS3)、白腐菌(White-rot fungi NS4)和绿色木霉(T.viride Persex Fx NS5)。经?瓶发酵和酶活检测,其羧甲基纤维素酶活(CMC酶活)分别为297.97 IU/mL,300.11 IU/mL,154.83 IU/mL,146.68 IU/mL,308.14 IU/mL;滤纸酶活(FPA酶活)分别为6.56 IU/mL,5.63 IU/mL,4.29 IU/mL,9.63 IU/mL,8.02 IU/mL。其中绿色木霉(T.viride PersexFxNS5)在发酵条件:麸皮:CMC-Na(1:1)各6 g/L,硫酸铵4 g/L,发酵温度30℃,发酵时间3 d,发酵液CMC酶活和滤纸酶活分别达到352.11 IU/mL和13.96 IU/mL,比初筛酶活分别提高14.26%和74.06%。

Screened by agar plate containing CMCNa and Congo red indicator,35 cellulase-producing strains were isolated from rotten wood,straw piles and soil sampled in NanChang.Based on their cellulase-producing activities,a rescreening was carried out and 5 cellulase-producing strains possesssing high CMCase and FPA activities were harvested.By the morphology analysis,such as colony,mycelium,spore,these 5 strains were identified as Ramularia NS1,Trichoderma koningii NS2,Penicillium glaucum NS3,White-rot fungi NS4 and T.viride Persex Fx NS5.When cultured in shake flasks,their CMCase activities could reach 297.97 IU/mL,300.11 IU/mL,154.83 IU/mL,146.68 IU/mL,308.14 IU/mL and the FPA activities of 6.56 IU/mL,5.63 IU/mL,4.29 IU/mL,9.63 IU/mL,8.02 IU/mL were achieved,respectively.Under the suitable fermentation conditions（CMC 6 g/L,wheat bran 6 g/L,（NH4）2SO4 4 g/L,at the temperature 30℃ for 3 days）,the yields of CMCase and FPAase produced by T.viride Persex Fx NS5 increased 14.26 % and 74.06 % than that of the original activities of 308.14 IU/mL and 8.02 IU/mL.