稻瘟菌侵染对水稻细胞骨架排列方式的影响

Effects of Magnaporthe grisea Penetration on Cytoskeleton Reorganization of Rice Cells

采用免疫荧光技术成功检测到水稻叶鞘内表皮细胞中微管和微丝的分布情况.稻瘟病菌的侵染使水稻叶鞘内表皮细胞中微管、微丝排列方式发生明显的改变,这种排列方式的改变非常灵敏,且在亲和性互作和非亲和性互作之间差异明显.非亲和性互作反应中,病菌侵染早期微管、微丝均放射状向病菌侵染点分布,至寄主细胞产生过敏性坏死时,逐渐受到破坏而降解.亲和性互作反应中,微管、微丝在病菌侵染的早期则已开始降解,形成短棒状或点状结构,比较均匀地分布于整个细胞,病原菌侵染菌丝在寄主细胞中扩展时,难以观察到完整的微管、微丝.

M. grisea penetration resulted in rapid and striking reorganization of microtubules and mierofilaments in cells of rice leaf sheath. The distribution patterns of cytoskeleton in the incompatible interaction of rice and M. grisea were different from those in compatible interaction. In cells undergoing resistant response, microtubules and microfilaments of rice cells radiated to fungal penetration site during the process of fungal penetration. Then they were gradually fragmented and eliminated from host cells after hypersensitive cell death occurred. However, in cells undergoing susceptible response, the cytoskeleton was severely damaged and fragmented quickly at the initial stage of fungal penetration. As the disease development, fine bundles of cytoskeleton were seldom observed in host cells although the cytoskeleton of M. grisea was normally detected.