摘要
目的探讨应力刺激在肩袖损伤术后腱-骨修复中的作用。方法选取18只成年雄性新西兰白兔,术前随机选取2只白兔处死,以熟悉解剖结构及对照用。将其余16只白兔随机分为两组,即应力刺激组(A组)与非应力刺激组(B组),每组各8只。A组术后第2周开始被动训练(CPM);B组正常笼养。分别于术后2、4、6、8周训练结束后处死,取材,每次每组各处死2只,检测BMP-2表达。结果术后2周,两组BMP-2染色均呈阳性,组间比较无明显差别。术后4周,两组BMP-2染色阳性,A组稍显均匀。术后6周,两组BMP-2染色阳性分布均较前广泛,A组较B组明显,且相对均匀有序。术后8周,A组比B组BMP-2染色更为有序,靠近骨面BMP-2表达程度与靠近腱性部分比较,差别较前减小。结论 CPM训练能提高兔冈上肌腱急性断裂术后腱-骨界面BMP-2表达,从而增进胶原的合成,促进肩袖损伤术后腱-骨修复。
Objective To study the impact of CPM to BMP-2 expression in repairing tendon-bone after operation to rabbit supraspinatus tendon of acute ruptur,and to explore the role of stress in the postoperative repairation after acute rotator cuff injury. Methods 18 adult male New Zealand white rabbits,two rabbits were randomly selected and had been put to death before operation （to become familiar with the normal anatomical structure and be reserved for the control samples available）. Another 16 rabbits were randomly divided into two groups：CPM group and non CPM group（nl =n2=8）. 2 weeks after operation,CPM group start to be trained;non-CPM group were normally feeded. After 2,4,6,8 weeks,respectively,2 rabbits each time in each group were put to death,drawn the materials and observed the changes of BMP-2 expression. Results After 2 weeks. BMP-2 expression of two groups also stained color, but no significant difference; after 4 weeks, BMP-2 expression of two groups also stained color, color in A group was slightly even; after 6 weeks, BMP-2 stained wider than before, For A group, it was even and order relatively; after 8 weeks,For A group,BMP-2 staining was more orderer than before,between closing to the bone and to the tendon,the difference of expression of BMP-2 decreased than before. Conclusion CPM can promote BMP2 expression in repairing of tendon-bone after op- eration to rabbit supraspinatus tendon of acute rupture, thereby further collagen synthesis, contribute to the recovery of tendon-bone after rotator cuff injury.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第15期1985-1987,共3页
Chongqing medicine
