镰形棘豆抗炎抗氧化活性研究及抗炎机制初探(英文)

Anti-inflammatory and Antioxidant Activities of Oxytropis falcata Fractions and Its Possible Anti-inflammatory Mechanism

目的:对镰形棘豆总生物碱、总挥发油、总黄酮、总皂苷和总多糖部位进行了体内外抗炎及抗氧化活性研究。方法:采用两种抗炎动物模型(TPA诱导的小鼠耳肿胀和卡拉胶诱导的小鼠腹腔白细胞迁移),评价镰形棘豆提取物的抗炎活性;以总抗氧化活性、DPPH、羟基自由基清除实验作为抗氧化性指标,评价镰形棘豆提取物的抗氧化活性,筛选镰形棘豆抗炎抗氧化活性部位。并采用急性和亚急性炎症模型探讨镰形棘豆总黄酮部位的抗炎作用机制,同时对其胃肠道保护作用进行了评价。结果:实验结果表明,镰形棘豆总黄酮部位具有较好的抗炎抗氧化活性,且无任何胃肠道刺激作用。其抗炎机制可能与抑制炎症介质PGE2的水平、降低机体脂质过氧化程度(MDA),提高机体抗氧化酶活性(SOD,CAT和GSH-Px)有关。结论:本研究为阐明传统药用植物镰形棘豆的抗炎作用提供科学的依据。

AIM：Alkaloid（AF）,essential oil（EOF）,flavonoid（FF）,saponin（SF） and polysaccharide（PF） fractions obtained from Oxytropis falcata Bunge were investigated for their anti-inflammatory and antioxidant activities in vivo and in vitro for the first time.METHOD：Two anti-inflammatory animal models（TPA-and carrageenin-induced） and three antioxidant models（total antioxidant activity,DPPH and hydroxyl radical-scavenging activity） were employed to evaluate the anti-inflammatory and antioxidant activities of O.falcata fractions.Animal models of acute inflammation and subacute inflammation were adopted to probe the possible anti-inflammatory mechanism.RESULTS：Flavonoids were major active constituents responsible for the anti-inflammatory and antioxidant activities of O.falcata.The anti-inflammatory mechanism of FF may be due,at least in part,to the inhibition of PGE2 levels,decrease of MDA levels and enhancement of SOD,CAT and GSH-Px activities without inducing any gastric damage.CONCLUSION：The results provide evidence to support the folk medicinal utilization of O.falcata for the treatment of inflammation.