肠必清栓对UC模型大鼠肠黏膜内IL-1β、TNF-α及SOD的影响

Effects of Changbiqing Suppository on the expression of IL-1β,TNF-α and SOD in colonic mucosa of rats with ulcerative colitis

目的:通过检测肠必清栓治疗后UC模型大鼠肠组织中IL-1β、TNF-α、SOD表达的变化,研究肠必清栓治疗UC的作用机制。方法:用2,4-二硝基氯苯免疫加醋酸局部灌肠法建立的UC模型大鼠48只随机分为五组,模型对照组(A组,8只),肠必清栓低、中、高剂量组(B、C、D组,各10只),柳氮磺吡啶药物对照组(E组,10只),另设正常对照组(F组,6只)。给药治疗14d后处死,留取结肠检测组织中细胞因子IL-1β、TNF-α、SOD的表达。结果:模型组大鼠肠组织中IL-1β、TNF-α表达显著增多(P<0.01),而SOD显著减少(P<0.01),给药治疗可下调IL-1β、TNF-α的表达,提高组织中SOD的表达。IL-1β、TNF-α的表达在肠必清栓低、中、高剂量组间呈剂量依赖关系,高剂量组与柳氮磺吡啶药物对照组间差异无统计学意义(P>0.05);SOD的表达在肠必清栓中、高剂量组、柳氮磺吡啶药物对照组和正常对照组间差异无统计学意义(P>0.05)。结论:肠必清栓可下调结肠组织中促炎性细胞因子的表达,提高组织SOD活性,这可能为其治疗UC调节免疫抗感染、抗氧化的作用机制。

Objective：To observe the effect of Changbiqing Suppository on the expression of IL-1β,TNF-α and SOD in colonic mucosa of rats with ulcerative colitis to reveal the possible mechanism for treatment.Methods：Fourty eight SD UC model rats induced by dinitrochlorobenzene（DNCB） and acetic acid were randomly assigned into five groups,UC model group（group A）,low,moderate and large dose intervention group（group B,C,D）,SASP group（group E）,another six rats served as normal control group（group F）.The fresh colons of the rats were got after 14 days of treatment and the antigen expression of IL-1β,TNF-α and SOD were evaluated.Results：The antigen expression of IL-1β,TNF-α in the model group was significantly higher（P0.01）,and expression of SOD was lower than the control group（P0.01）.Compared with group A,the expression of IL-1β,TNF-α in group B to group E decreased markedly and the expression of SOD increased significantly.The change of IL-1β,TNF-α were in a dose-dependent manner in group B to group D,no significant difference was observed for the antigen expression of SOD among group C,group D,group E and group F.Conclusion：Changbiqing Suppository can adjust immunization anti-inflammatory,anti-oxidation,this may be the mechanism for its treatment of UC.