促红细胞生成素对β淀粉样蛋白诱导大鼠海马CA_1区细胞凋亡的保护作用及机制

The protective effect of EPO on rat hippocampus cell apoptosis induced with amyloid beta-protein and the mechanism

目的探讨促红细胞生成素(Erythropoietin,EPO)对阿尔茨海默病(Alzheimer disease,AD)大鼠脑损伤的保护机制。方法采用大鼠海马内注射β淀粉样蛋白制作AD模型。将SD大鼠随机分为假手术对照组、生理盐水对照组及EPO处理组。大鼠海马内注射Aβ1-40造模,然后在生理盐水对照组大鼠行脑室立体定向注射生理盐水,EPO处理组则行脑室立体定向注射重组人促红细胞生成素(rHu-EPO)。观察手术后24h大鼠海马CA1区抗凋亡蛋白Bcl-xl表达变化,以及术后7d海马CA1区细胞凋亡变化。结果 EPO处理组和生理盐水组海马CA1区大鼠Bcl-xl蛋白表达较假手术对照组减少,但是EPO处理组Bcl-xl蛋白表达高于生理盐水(P<0.05)。生理盐水组海马CA1区凋亡细胞明显多于EPO组(P<0.05)。结论 EPO可以抑制β淀粉样蛋白诱导海马CA1区细胞凋亡,与其抑制Bcl-xl蛋白表达下降有关。

Objective To explore the protection mechanism of erythropoietin （EPO） on Alzheimer disease （AD） rat brain injury.Methods With amyloid beta-protein（Aβ） injection into rat hippocampus,we made AD model.Male Spraque-Dawley rats were randomly separated into 3 groups including sham control,saline control and EPO treatment.After Aβ1-40 injected into rats hippocampus,saline or EPO was respectively injected into the lateral ventricle of rats,with the help of stereotaxic coordinates,upon the designed conditions.Hippocampal CA1 subregion Bcl-xl expression Changes were observed 24h after the operation,and apoptosis in hippocampus CA1 subregion were observed 7d after the operation.Results The EPO group had better Bcl-xl expression than the saline group（P〈0.05）and hippocampal CA1 subregion showed the stronger distribution of apoptosis in saline group than in EPO group（P〈0.05）.Conclusions EPO could restrain hippocampal CA1 subregion apoptosis in the modeled rats,and it could be related with EPO restraining Bcl-xl expression decreasing.