米诺环素对实验性自身免疫性脑脊髓炎大鼠凋亡相关蛋白表达的影响

Effect of minocycline on expression of apoptosis-related proteins in rats with experimental autoimmune encephalomyelitis

目的探讨米诺环素对实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)大鼠CNS内Fas和Bcl-2蛋白表达的影响。方法将大鼠随机分为3组,并以豚鼠全脊髓匀浆(guinea pig spinal cord homogenate,GPSCH)为抗原免疫建立EAE模型。治疗组给予米诺环素,而对照组和模型组给予生理盐水灌胃;免疫组化检测Fas和Bcl-2的表达。结果 Fas的表达各组平均光密度和阳性细胞数模型组高于治疗组(P<0.05);Bcl-2的表达除脑干阳性细胞数外,平均光密度及细胞数治疗组高于模型组,对照组低于模型组(P<0.05)。结论米诺环素可能通过介导Fas和Bcl-2的表达,干预凋亡调节免疫反应对EAE起治疗作用。

Objective To investigate the effect of minocycline on expression of Fas and Bcl-2 in CNS of rats with experimental autoimmune encephalomyelitis （EAE）.Methods Rats were divided into three groups randomly and EAE was induced in rats by immunization with guinea pig spinal cord homogenate （GPSCH）.The rats in the treatment group were given minocycline （90mg/kg,on the first day;45mg/kg,on the following days） by gavage,and rats in the control and model groups were given normal saline by gavage.The expression of Fas and Bcl-2 in the CNS were estimated by immunohistochemical method.Results The expression of Fas： compared with treatment group,the average density and positive cells were higher in every part of model group（P0.05）;The expression of Bcl-2：the average density and positive cells of treatment group were more than model group in every part except brain stem,and the control group was lower than model group（P0.05）.Conclusion Minocycline may be able to regulate the expression of Fas and Bcl-2 proteins,and have influence on apoptosis in EAE and may have immunoregulatory actions on EAE.