骨髓基质细胞源神经干细胞对血管性痴呆大鼠行为及血管内皮生长因子蛋白表达的影响

Effects of bone marrow stromal cells derived neural stem cells on the dynamic changes of behavior and expression of VEGF protein in vascular dementia rats

目的探讨骨髓基质细胞(BMSCs)源神经干细胞对血管性痴呆大鼠行为及血管内皮生长因子(VEGF)蛋白表达的影响。方法建立大鼠血管性痴呆模型。32只健康Sprague-Dawley(SD)大鼠分为假手术组、缺血对照组、BMSCs移植组和BMSCs源神经干细胞移植组。于移植后30d对大鼠进行学习记忆能力检测,并应用免疫组化检测上皮干细胞蛋白(Nestin)和VEGF蛋白表达。结果 BMSCs源神经干细胞移植组、BMSCs移植组、血管性痴呆对照组大鼠学习记忆能力较假手术组下降。移植后30d各组神经功能均有不同程度的恢复,BMSCs源神经干细胞移植组大鼠的学习记忆能力显著优于BMSCs移植组,差别有统计学意义(P<0.01);BMSCs源神经干细胞移植组Nestine和VEGF阳性细胞多于BMSCs移植组,差别有统计学意义(P<0.01)。结论 BMSCs源神经干细胞移植可改善大鼠学习记忆能力、促进神经干细胞的增殖、增加VEGF蛋白表达,显著优于BMSCs移植。

Objective To study the effects of bone marrow stromal cells （BMSCs） derived neural stem cells on behavior and expression of VEGF protein in vascular dementia rats.Methods The model of vascular dementia were established.32 Sprague-Dawley rats were divided into sham-operated group, vascular dementia control group,BMSCs transplanted group and BMSCs derived neural stem cells transplanted group.The learning and memory ability of the rats were evaluated on 30d after transplantation.The rats were killed separately on 30d after operation. The brain sections were used for Nestine and VEGF staining by immunohistochemistry methods.Results Learning and memory ability of BMSCs derived neural stem cells transplanted group,BMSCs transplanted group and vascular dementia control group decreased than those of sham-operated group. The recovery of learning and memory ability in BMSCs derived neural stem cells transplantation group was evidently than BMSCs transplantation group on 30d after transplantation.The number of Nestine and VEGF positive cells in BMSCs derived neural stem cells transplantation group increased evidently compared with that of the BMSCs transp lantation group.Conclusion BMSCs derived neural stem cells can effectively improve the learning and memory ability, improve neural cells proliferation and increase the expression of VEGF protein in VD rats.