T-AK细胞的细胞毒活性研究

Study on cytotoxicity of human T activated killer cells

目的：探讨抗ＣＤ３单抗（ＣＤ３ＭｃＡｂ）和ｒＩＬ２共同激活诱生的ＴＡＫ（Ｔａｃｔｉｖａｔｅｄｋｉｌｅｒ）细胞的细胞毒活性本质。方法：采用ＭＴＴ法分别检测ＴＡＫ细胞杀伤白血病细胞的活性及其构成。结果：ＴＡＫ细胞杀伤白血病细胞的活性主要由４部分组成：ＣＤ３ＭｃＡｂ激活的ＣＤ３ＡＫ活性约占５０％，ｒＩＬ２诱生的ＬＡＫ细胞活性约为３０％，ＮＫ活性约占１０％，可溶性抑制性因子的抑制活性约为１０％；细胞表型分析ＴＡＫ细胞主要表达活化Ｔ淋巴细胞和ＮＫ细胞的表面标志。结论：ＴＡＫ细胞为异质性细胞群体。

Objective:To study the essence of the cytotoxicity of T activated killer (T AK) cells costimulated by anti CD3 monoclonal antibody (CD3McAb) and recombinant interleukin 2 (rIL 2).Methods:T AK activity against leukemia cells (K 562 cells and Raji cells) were determined separately in a MTT assay.Results:The cytotoxicity of T AK cells were composed of mainly four parts:CD3 McAb activated CD3AK activity(50%),rIL 2 induced LAK activity(30%),NK activity(10%) and the activities of inhibitory factors in T AK supernatant(10%).The analyses of phenotypes showed that T AK cells mainly expressed the surface makers of activated T lymphocytes and NK like cells.Conclusion:T AK cells are heterogeneous cell population,consisting of mainly activated T lymphocytes and NK like cells,the main part of T AK cytotoxicyty is CD3 AK activity and LAK activity.