巨噬细胞诱发的实验性增生性玻璃体视网膜病变中的几种早期炎性细胞因子

TNF α, IL 8 and IL 6 in the early inflammatory stage of experimental PVR model induced by macrophages

目的观察在增生性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）模型中几种早期炎性细胞因子的含量变化及其与ＰＶＲ形成时相的关系。方法采集巨噬细胞诱发的兔眼ＰＶＲ模型的玻璃体液用双抗体夹心法酶联免疫吸附测试（ｅｎｚｙｍｅｌｉｎｋｅｄｉｍｍｕｎｏｓｏｒｂｅｎｔａｓａｙ，ＥＬＩＳＡ）试剂盒，检测样本中肿瘤坏死因子α（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒα，ＴＮＦα）、白细胞介素８（ｉｎｔｅｒｌｅｕｋｉｎ８，ＩＬ８）和白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ６）的含量，并进行多元回归拟合曲线分析。结果ＴＮＦα在巨噬细胞注入后７天上升，２１天达高峰值，为３０９ｐｇ／ｍｌ；ＩＬ８与ＩＬ６在７～２１天也维持高水平，在１４天分别达高峰值１３２５ｐｇ／ｍｌ和９９８ｐｇ／ｍｌ。与对照组比较差异均有非常显著性。结论３种早期炎性细胞因子ＴＮＦα、ＩＬ８和ＩＬ６在本模型的炎症期和细胞增生期均呈明显的高水平，在瘢痕期水平降低，提示它们对眼内细胞增生具有重要的启动和调控作用。

Objective To observe the levels of cytokines, tumor necrosis factor α (TNF α), interleukin 8 (IL 8) and IL 6 in the early inflammatory stages of a rabbit proliferative vitreoretinopathy (PVR) model induced by macrophages. Methods The vitreous and venous blood of rabbit eyes of the PVR model were obtained and tested with ELISA kits of TNF α, IL 8 and IL 6 and analyzed statistically. Results The TNF α level increased at the 7th day after macrophage injection and reached its peak (309 pg/ml) on day 21. IL 8 and IL 6 maintained their higher levels during 7 to 21 days after macrophage injection and the peak levels were 1 325 pg/ml and 998 pg/ml on day 14 respectively. Those showed significant differences compared to control ( P ≤0.05). Conclusion The 3 cytokines, TNF α, IL 8 and IL 6, present high levels in the early stage and proliferative phase of the PVR model and decrease to normal level in the regeneration (scarring) phase, indicating that these cytokines may play an important role in initiating and modulating the cellular proliferation.