摘要
目的:建立一种测定清消口服液中绿原酸含量的方法。方法:RPHPLC法。采用HypersitC18柱(5μm,3.9×150mm),甲醇水冰醋酸(17∶180∶3.1)为流动相,流速1.4ml·min-1,紫外检测波长327nm。结果:线性范围0.064~1.28μg,r=0.9995,回收率在99%以上。结论:本法测定清消口服液中绿原酸的含量,操作简便,结果准确。
OBJECTIVE:To establish a method for the determination of the Chlorogenic acid in Qingxiao oral solution.METHODS:The Chlorogenic acid was seperated on a Hypersil ODS C 18 column( 3.9 ×150 mm) and detected at 327 nm,using methanol water acetic acid(17∶180∶ 3.1 ) as the mobile phase.RESULTS:The calibration curve was linear( r = 0.999 5 ) within the range of 64 ng to 1 280 ng.The average recovery was more than 99%.CONCLUSIONS:The method was sample and accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1999年第3期144-145,共2页
Chinese Journal of Hospital Pharmacy