簇毛麦(Dasypyrum villosum L.)中一个γ-醇溶蛋白基因序列的研究

Sequence of a New γ-gliadin Gene in Dasypyrum villosum L.

目的:研究簇毛麦中一个新的γ-醇溶蛋白基因序列及结构特点,为小麦品质育种和该类基因的进化分析提供资料。方法:利用基因组PCR技术从簇毛麦基因组中克隆到1个γ-醇溶蛋白基因(Dv-γ)的全编码序列,利用生物信息学研究其序列结构特点。结果:该序列与已知的γ-醇溶蛋白有着很高的相似性。推导的氨基酸序列包含5个典型的结构域,其中含有8个保守半胱氨酸残基,高变重复区Ⅱ中的重复单元与其他物种来源基因有较大区别。γ-醇溶蛋白中常见的乳糜泄抗原决定簇在其内部也有发现。进化分析表明,此基因与亚家族Ⅰ中的γ-醇溶蛋白基因有着较近的亲缘关系。结论:获得了一个新的γ-醇溶蛋白基因。

Objective：A new γ-gliadin gene structure from Dasypyrum villosum L.was studied in order to provide new data for wheat breeding and γ-gliadin evolution.Method：The full open reading frame of this gene was cloned using a genomic PCR-based strategy and named Dv-γ,and its characters were studied by bio-informatic technique.Result：Sequence analyses showed that it was similar to those previously reported.The primary structure of the putative protein comprised five domains and contained eight cysteine residues.There were significant differences in domain Ⅱ comparing Dv-γ from Dasypyrum villosum L.to γ-gliadins from other species.Coeliac disease epitopes were also found in this gene.Phylogenetic analyses showed that the mature γ-gliadin sequence fell into sub-family I of γ-gliadins.Conclusion：A new γ-gliadin gene was acquired.