肠道病毒EV71感染对T细胞免疫功能影响的研究

The Effects of Enterovirus 71 Infection on the Ability of T Cellular Immunity

目的探讨肠道病毒EV71感染对T淋巴细胞系Jurkat细胞免疫功能的影响。方法首先,用RT-PCR和免疫荧光的方法检测EV71能否感染Jurkat细胞,其次用Annexin V-FITC试剂检测EV71感染后的Jurkat细胞凋亡的情况,最后用ELISA的方法分析感染后的Jurkat细胞分泌细胞因子的情况。结果 EV71能够感染Jurkat细胞,并能够引起Jurkat细胞的凋亡,同时EV71感染还能导致Jurkat细胞分泌细胞因子IL-2,IFN-γ,IL-6和IL-10的能力下降。结论 EV71病毒感染对机体免疫功能的影响是多方面的,它不但能够诱导T细胞的凋亡,还能引起T细胞分泌细胞因子的能力降低,从而在数量和功能上影响参与免疫应答的T淋巴细胞。

Objective To investigate the effects of Enterovirus 71 infection on the immune function of Jurkat cells. Methods First, the fact that Jurkat cells could be infected by EVT1 had been detected by RT -PCR and immunofluorescence. Second, the apoptosis of Jurkat cells after EVT1 infection was detected by Annexin V -FITC. Third, the cytokine secretion ability of Jurkat cells was analyzed by ELISA assay. Results EV71 could infect Jurkat cells. The infection could induce apoptosis. Meanwhile, the ability of Jurkat cells to secret cytokine include IL - 2, IFN -γ, IL - 6 and IL - 10 could also be weakened by the infection of EV71. Conclusion The immune function could be extensively affected after EVT1 infection. Not only the apoptosis but also the cytokine secretion decreasing could be induced by EVT1 infection. In summary, the quantities and function of those T lymphocytes, which was involved in the immune response, could be affected by EVT1 infection.