大鼠乙酸胃溃疡愈合过程中EGF和VEGF对胃黏膜组织形态的影响

The Effect of EGF and VEGF on Morphology of Gastric Mucosal Tissue in Healing Process of Acetic-acid-induced Gastric Ulcer in Rats

目的探讨表皮生长因子(epidermal growth factor,EGF)和血管内皮生长因子(vascular endothelial growth factor,VEGF)对再生胃黏膜形态的影响。方法采用大鼠乙酸溃疡模型,用免疫组化方法观察了EGF和VEGF在溃疡3天、8天、25天时的表达,同时测定了再生黏膜厚度、腺体扩张数、胃黏液量,并分析了EGF和VEGF对再生黏膜厚度、腺体扩张数、胃黏液量的影响。结果 25天组的EGF积分光密度高于8天组(P<0.01),8天组的积分光密度高于3天组(P<0.05);25天组的VEGF积分光密度高于8天组(P<0.01),8天组的积分光密度高于3天组(P<0.01);25天组的再生黏膜厚度高于8天组(P<0.01),8天组的再生黏膜厚度高于3天组(P<0.01);25天组的腺体扩张数低于8天组(P<0.01),8天组的腺体扩张数低于3天组(P<0.01);25天组的胃黏液含量高于8天组(P<0.01),8天组的胃黏液含量高于3天组(P<0.01)。结论随着溃疡的愈合,EGF和VEGF促进了再生的异常胃黏膜向正常胃黏膜转化,并促进了再生胃黏膜功能的恢复。

Objective To explore the effect of EGF and VEGF on morphology of regenerative gastric mucosa. Methods Gastric ulcers were induced with acetic acid in rats, and the expression of EGF and VEGF was observed with immunobistochemistry method on day 3, 8 and 25 following ulcer induction. The regenerative mueosal thickness, the number of dilated gland and the volume of gastric mucus were also measured on day 3,8 and 25 following ulcer induction. And the effects of EGF and VEGF on the regenerative mucosal thickness, the number of dilated gland and the volume of gastric mucus were analyzed. Results The integral light density of EGF on day 25 was more than that of EGF on day 8 （ P 〈 0.01 ）. The integral light density of EGF on 8 day was more than that of EGF on day 3 （ P 〈 0.05 ）.The integral light density of VEGF on day 25 was more than that of VEGF on day 8 （ P 〈 0.01 ）. The integral light density of VEGF on 8 day was more than that of VEGF on day 3 （P 〈 0.01 ）. The regenerative mucosal thickness on day 25 was more than that on day 8 （ P 〈 0.01 ）. The regenerative mucosal thickness on day 8 was more than that on day 3 （ P 〈 0.01 ）. The number of dilated gland on day 25 was less than that on day 8. The number of dilated gland on day 8 was less than that on day 3（P 〈0.01 ）. The volume of gastric mucus on day 25 was more that on day 8 （ P 〈 0.01 ）. The volume of gastric mucus on day 8 was more than that on day 3 （ P 〈 0.01 ）. Conclusion With the ulcer healing, EGF and VEGF make regenerative deformans gastric mucosa convert to normal gastric mucosa, and make regenerative gastric mucosa restore its functions.