蝎毒抗癌多肽对移植性肿瘤的抑制作用

Inhibitory effect of antineoplastic polypeptide from Buthus martensii venom on transplanting tumors

目的：观察蝎毒抗癌多肽（ａｎｔｉｎｅｏｐｌａｓｔｉｃｐｏｌｙｐｅｐｔｉｄｅｆｒｏｍＢｕｔｈｕｓｍａｒｔｅｎｓｉｖｅｎｏｍ，ＡＰＢＭＶ）对小鼠肝癌（Ｈｅｐａｔｏｍａ２２，Ｈ２２）和小鼠黑色素瘤（ｍｅｌａｎｏｍａＢ１６）两种动物移植性肿瘤的抑制作用。方法：采用肿瘤质量和肿瘤生长抑制以及带瘤小鼠外周血白细胞计数和脾脏指数为观察指标。将接种Ｈ２２和Ｂ１６２４ｈ后的带瘤小鼠分为对照组，５Ｆｕ治疗组和ＡＰＢＭＶ的３个治疗组。ＡＰＢＭＶ治疗组小鼠分别给予ＡＰＢＭＶ０．０３ｍｇ·ｋｇ－１，０．０４ｍｇ·ｋｇ－１和０．０６ｍｇ·ｋｇ－１腹腔注射，５Ｆｕ组和对照组分别给予５Ｆｕ２０ｍｇ·ｋｇ－１和等体积生理盐水；给药１０ｄ后处死带瘤小鼠，取实体瘤称重并计算肿瘤生长抑制率。Ｈ２２带瘤小鼠于处死前眼球取血，计数外周血细胞，并取小鼠脾脏，称重，计算脾脏指数（ｓｐｌｅｅｎｉｎｄｅｘ，ＳＩ）。结果：ＡＰＢＭＶ在应用的３个剂量范围内对Ｈ２２均具有明显的抑制作用，抑制率均＞３０％，与对照组相比差异显著（Ｐ＜０．００１）；ＡＰＢＭＶ的中、高剂量对Ｂ１６具有显著的抑制作用，抑制率分别为４０．３６％和４４．５８％，其对Ｂ１６的抑制作用强于５?

Aim:To observe the inhibitory effect of antineoplastic polypeptide from Buthus martensii venom (APBMV)on transplanting tumors hepatoma 22(H 22 ) and melanoma B 16 . Methods:Tumor weight,tumor growth inhibitory rate(IR%), WBC count in peripheral blood and spleen index(SI) measuring of tumor bearing mice were used as the indexes.Mice were inoculated by H 22 and B 16 ,and 24 h later the tumor bearin gmice were divided into control, 5 Fu treated and the APBMV treated groups.The mice in APBMV treated group were administered 0.03 mg·kg -1 ,0.04 mg·kg -1 and 0.06 mg·kg -1 of APBMV separately by intraperitoneal injection. 5 Fu treated and control group were given 20 mg·kg -1 of 5 Fu and equal volume saline,respectivety.Tumor bearing mice were killed after the chemicals were given for 10 days. Results:Growth of H 22 was inhibited markedly by APBMV at three doses used in this test.Inhibitory rate was over 30%compared with control group and there was a significant difference ( P <0.001).B 16 growth was inhibited by two high doses of APBMV,inhibitory rates were 40.36%and 44.58%respectively.Inhibitory effect of APBMV on B 16 was stronger than 5 Fu(33.55%). Compared with control, WBC count and SI of H 22 bearing mice were reduced notably. There were significant differences between 5 Fu and controla and APBMV treated groups( P <0.001).Conclusion:Growth of H 22 and B 16 tumors was efficiently inhibited by APBMV and it also conduces to the maintenance of normal function of tumor bearing mice.