摘要
目的:观察蝎毒抗癌多肽(antineoplasticpolypeptidefromButhusmartensivenom,APBMV)对肝癌H22带瘤小鼠NK细胞活性,外周血白细胞计数,淋巴细胞增殖,迟发型超敏反应和血清溶血素水平的影响。方法:将接种H22细胞24h后的带瘤小鼠随机分为4组,每组10只:空白对照组(等体积生理盐水,腹腔注射),5Fu组(5Fu10mg·kg-1),APBMV组(APBMV003mg·kg-1,腹腔注射),APBMV+5Fu组(APBMV003mg·kg-1+5Fu10mg·kg-1,腹腔注射),连续给药5d。给药期间及给药后对带瘤小鼠进行相应的处理,观察经治疗后H22带瘤小鼠上述指标的变化。结果:APBMV组及APBMV+5Fu组带瘤小鼠的NK细胞活性和DNCB诱导的皮肤迟发型超敏反应明显增强,淋巴细胞转化指数明显增高,与对照组及单用5Fu组相比有显著差异(P<005或P<0.01或P<0.001);APBMV单独应用使带瘤小鼠白细胞计数显著提高(与对照组相比P<001),5Fu组白细胞计数明显减少(与对照相比P<001),APBMV与5Fu联合应用?
Aim:To observe
the influence of antineoplastic polypeptide from Buthus martensii venom(APBMV) on NK
activity,WBC of peripheral blood,lymphocyte blastgenesis,delayed type hypersensitivity(DTH)
response and homolysin evels of hepatoma H 22 bearing mice.Methods: The mice
inoculated by hepatoma H 22 cells for 24 h were divided into 4 groups of 10 mice each.Mice
in control group were intraperitoneally(ip.)given saline.Mice were ip injected 5 Fu at 10 mg·kg
-1 in 5 Fu treated group.In APBMV treated group mice were ip administered APBMV 0.03
mg·kg -1 . In combined APBMV with 5 Fu (APBMV+5 Fu) group mice were ip administered
APBMV(0.03 mg·kg -1 )and 5 Fu (10 mg·kg -1 )together. All chemicals were given to
mice for 5 days.The changes of indexes mentioned above of H 22 carrying mice were
observed after treatments.Results:NK cells activity and response of DHT induced by DNCB in H
22 carrying mice enhanced remarkably and proliferating index of lympocyte activited by ConA
increased evidently in both APBMV and APBMV+5 Fu groups.Compared with control and 5 Fu
groups there were significant differences( P <0.05 or P <0.01 or P <0.001).WBC number of H
22 bearing mice treated with APBMV alone increased markedly compared with control ( P
<0.01).WBC count decreased markedly( P <0.01) in the mice treated with 5 Fu compared with
control,but in APBMV+5 Fu group, WBC count increased approaching the control level. The
difference of WBC count between APBMV+5 Fu and 5 Fu groups was significant( P
<0.05).APBMV could also enhance the homolysin level of peripheral blood in H 22 bearing
mice( P <0.05).Conclusion:APBMV can antagonize or recover the immunity inhibition or defect
induced by chemical drugs or the tumor.
出处
《河南医科大学学报》
1999年第1期67-70,共4页
Journal of Henan Medical University
基金
河南省"九五"科技攻关项目
关键词
肝肿瘤
免疫功能
APBMV
小鼠
Scorpion venom
transplanting
tumor
immunity
polypeptide