蝎毒抗癌多肽对肝癌小鼠免疫功能的影响

Influence of antineoplastic polypeptide from Buthus martensii venom on immune system function of hepatoma H 22 bearing mice

目的：观察蝎毒抗癌多肽（ａｎｔｉｎｅｏｐｌａｓｔｉｃｐｏｌｙｐｅｐｔｉｄｅｆｒｏｍＢｕｔｈｕｓｍａｒｔｅｎｓｉｖｅｎｏｍ，ＡＰＢＭＶ）对肝癌Ｈ２２带瘤小鼠ＮＫ细胞活性，外周血白细胞计数，淋巴细胞增殖，迟发型超敏反应和血清溶血素水平的影响。方法：将接种Ｈ２２细胞２４ｈ后的带瘤小鼠随机分为４组，每组１０只：空白对照组（等体积生理盐水，腹腔注射），５Ｆｕ组（５Ｆｕ１０ｍｇ·ｋｇ－１），ＡＰＢＭＶ组（ＡＰＢＭＶ００３ｍｇ·ｋｇ－１，腹腔注射），ＡＰＢＭＶ＋５Ｆｕ组（ＡＰＢＭＶ００３ｍｇ·ｋｇ－１＋５Ｆｕ１０ｍｇ·ｋｇ－１，腹腔注射），连续给药５ｄ。给药期间及给药后对带瘤小鼠进行相应的处理，观察经治疗后Ｈ２２带瘤小鼠上述指标的变化。结果：ＡＰＢＭＶ组及ＡＰＢＭＶ＋５Ｆｕ组带瘤小鼠的ＮＫ细胞活性和ＤＮＣＢ诱导的皮肤迟发型超敏反应明显增强，淋巴细胞转化指数明显增高，与对照组及单用５Ｆｕ组相比有显著差异（Ｐ＜００５或Ｐ＜０．０１或Ｐ＜０．００１）；ＡＰＢＭＶ单独应用使带瘤小鼠白细胞计数显著提高（与对照组相比Ｐ＜００１），５Ｆｕ组白细胞计数明显减少（与对照相比Ｐ＜００１），ＡＰＢＭＶ与５Ｆｕ联合应用?

Aim:To observe the influence of antineoplastic polypeptide from Buthus martensii venom(APBMV) on NK activity,WBC of peripheral blood,lymphocyte blastgenesis,delayed type hypersensitivity(DTH) response and homolysin evels of hepatoma H 22 bearing mice.Methods: The mice inoculated by hepatoma H 22 cells for 24 h were divided into 4 groups of 10 mice each.Mice in control group were intraperitoneally(ip.)given saline.Mice were ip injected 5 Fu at 10 mg·kg -1 in 5 Fu treated group.In APBMV treated group mice were ip administered APBMV 0.03 mg·kg -1 . In combined APBMV with 5 Fu (APBMV+5 Fu) group mice were ip administered APBMV(0.03 mg·kg -1 )and 5 Fu (10 mg·kg -1 )together. All chemicals were given to mice for 5 days.The changes of indexes mentioned above of H 22 carrying mice were observed after treatments.Results:NK cells activity and response of DHT induced by DNCB in H 22 carrying mice enhanced remarkably and proliferating index of lympocyte activited by ConA increased evidently in both APBMV and APBMV+5 Fu groups.Compared with control and 5 Fu groups there were significant differences( P <0.05 or P <0.01 or P <0.001).WBC number of H 22 bearing mice treated with APBMV alone increased markedly compared with control ( P <0.01).WBC count decreased markedly( P <0.01) in the mice treated with 5 Fu compared with control,but in APBMV+5 Fu group, WBC count increased approaching the control level. The difference of WBC count between APBMV+5 Fu and 5 Fu groups was significant( P <0.05).APBMV could also enhance the homolysin level of peripheral blood in H 22 bearing mice( P <0.05).Conclusion:APBMV can antagonize or recover the immunity inhibition or defect induced by chemical drugs or the tumor.