肺癌组织MDR1和GST-πmRNA水平测定及其临床意义

Clinical Significance of Detection of MDR1 and GST -π mRNAin Lung Cancer Tissues

分析肺癌组织ＭＤＲ１和ＧＳＴ－π基因的表达和临床意义。方法：运用ＲＴ－ＰＣＲ方法对５１例手术切除肺癌组织和相应的癌周正常肺组织进行ＭＤＲ１和ＧＳＴ－πｍＲＮＡ检测。结果：ＭＤＲ１ｍＲＮＡ在正常肺组织和肺癌组织中表达阳性率分别为１３．７％（７／５１）和４３．１％（２２／５１）；而ＧＳＴ－πｍＲＮＡ阳性率则分别为１９．６％（１０／５１）和５１．０％（２６／５１）．ＭＤＲ１或ＧＳＴＤ－πｍＲＮＡ表达与肿瘤病理类型。组织分化、ＴＮＭ分期等均无明显的相关性（Ｐ＞０．０５）。１９例（３７．３％）发生ＭＤＲ１和ＧＳＴ－πｍＲＮＡ共表达；２２例（４３．１１％）二者均无表达；７例（１３．７％）仅表达ＧＳＴ－πｍＲＮＡ：３例（５．９％）仪表达ＭＤＲ１ｍＲＮＡ。结论：肺组织细胞在恶变过程中ＭＤＲ１和ＧＳＴ－πｍＲＮＡ表达均有所增加，二者在肺癌先天性耐药机制中均占有重要的作用，而且二者在肺癌中的表达基本是一致的。

The expression of MDR1 and GST -π gene in 51 lung specimens and normal tissues near-by the cancer were studied by reverse transcription - polymerase chain reaction (RT - PCR) . The re-sults showed that 7 of the 51 normal tissues ( 13. 7% ) and 22 of the 51 tumor specimens (43. 1% ) hadpositive expression of MDR1 gene ( P<0. 01) , while 10 of the 51 normal tissues ( 19. 6% ) and 26 ofthe 51 tumor specimens (51.0% ) expressed GST -πgene ( P < 0. 01) . No correlations were notedin between MDR1/GST -π gene expression with histological differentiation, pathological type andTNM stage. Nineteen of the 51 lung cancer specimens co - expressed MDR1 and GST - π gene in cancertissues ( P>0.05) . The results suggest that the level of MDR1 /GST - π gene expression in lung cellsincreases in the process of carcinogenesis. MDR1 and GST - π gene might be taken as tumor markers oflung cancer and that coexpression of MDR1 and GST -π gene is of significance in the mechanism ofdrug resistance of lung cancer.