尿路上皮肿瘤ras　p21和p53蛋白和DNA含量的流式细胞定量研究

Quantitative Study of ras p21, p53 Protein Expression and DNA Content in Urothelial Tumors

研究ｒａｓｐ２１和ｐ５３表达和ＤＮＡ倍体异常与尿路上皮肿瘤发生关系。方法：应用流式细胞术和细胞免疫荧光技术对５２例尿路上皮肿瘤ｒａｓｐ２１和ｐ５３蛋白和ＤＮＡ含量进行定量检测。结果：异倍体２２例（４２．３％），二倍体３０例（５７．７％）；异倍体肿瘤其ＤＮＡ指数、增殖指数和Ｓ期细胞比率均高于二倍体肿瘤，但差异无显著性（Ｐ＞０．０５）；ｒａｓｐ２１和ｐ５３共同表达２５例（４８．１％），随肿瘤病理分级分期的上升而增加（Ｐ＜０．０５）；异倍体肿瘤ｒａｓｐ２１和ｐ５３共同表达１６例（７２．７％），二倍体肿瘤ｒａｓｐ２１和ｐ５３共同阳性９例（３０％），两组间差异显著（Ｐ＜０．０１）。结论：ＤＮＡ倍体异常、ｒａｓｐ２１和ｐ５３的共同表达参与尿路上皮肿瘤的发生和发展。||关键词##4尿路上皮肿瘤;;ｒａｓｐ２１;;ｐ５３;;ＤＮＡ倍体;;

Objective: To study the relationship between the abnormal ras p21, p53 expression,DNA ploidy and the carcinogenesis of urothelial tumors. Methods: The ras p21, p53 protein expressionand DNA content were quantitatively deternlined using flow cytometry and cellular immunofluorescence tech-nique in paraffin embedded tissues from 52 cases of urothelial tumors. Results: 42. 3% (22/52) of aneu-ploidy and 57. 7% (30/52) of diploidy were observed in 52 cases of urothelial tumors. The DNA index,proliferation index and S - phase fraction in aneuploidy tumors were higher than those in the diploidy tu-more, but the difference was not significant ( P > 0. 05) . Co - positive expression of ras p21 and P53were seen in 25 cases (48. 1% ) and it increased along with the escalation of the pathological grading(P<0.05) . Coexpression of ras p21 and p53 observed in aneuploidy tumors was 72.7% ( 16/22)and in diploidy was 30% (9/30) with significant difference ( P<0.01) . Conclusion: DNA ploidyabnormality and co - expression of ras p21 and p53 play an important role in the development of urothe-lial tumors.