血管紧张素Ⅱ对血管内皮细胞整合素β3表达的影响

Effects of angiotensin Ⅱ on the expression of integrin β3 in the surface of vascular endothelial cell

目的和方法：本实验采用细胞ＥＬＩＳＡ和ＲＴ－ＰＣＲ的方法分别从蛋白质和ｍＲＮＡ水平对血管紧张素Ⅱ（Ａｎｇ－Ⅱ）作用下的内皮细胞表面整合素β３的表达进行检测。结果：１０－８ｍｏｌ／Ｌ～１０－５ｍｏｌ／Ｌ的Ａｎｇ－Ⅱ在蛋白质水平能够促进内皮细胞整合素β３表达（Ｐ＜０．０５），且呈剂量依赖性。１０－６ｍｏｌ／Ｌ的Ａｎｇ－Ⅱ作用培养的人脐静脉内皮细胞１８ｈ后，细胞整合素β３ｍＲＮＡ量为正常对照组的１５倍。结论：提示Ａｎｇ－Ⅱ促进内皮细胞表面整合素β３的表达可能是单核细胞和内皮细胞之间粘附功能增加的机制之一。

AIM and METHODS:This study was performed on endothelial cells(EC) to observe the effect of angiotension Ⅱ(Ang-Ⅱ) on the expression of integrin β3 by using cell-ELISA and RT-PCR analyses.RESULTS:Stimulation of EC with Ang-Ⅱ resulted in dose-dependent increase in expression of integrin β3 chains in the surface of EC. RT-PCR analysis demonstrated that a 1.5 fold incresase in Ang-Ⅱ (10 -6 mol/L) induced expression of mRNA for β3 integrin.CONCLUSION:The protein and mRNA expression of integrin β3 on EC were regulated by Ang-Ⅱ, and that this regulation may be important in cell adherence, and in the pathophysiology of atherosclerosis.