Ⅰ型胶原与氧化性低密度脂蛋白相互作用的机制研究

Studies on the mechanism of the interaction between type I collagen and oxidative low density lipoprotein

目的：阐明胶原与血清低密度脂蛋白（ＬＤＬ）之间的关系。方法：在体外制备了Ⅰ型胶原凝胶，观察可能影响氧化ＬＤＬ（ｏｘ－ＬＤＬ）与胶原结合的几种因素。结果：随着ＬＤＬ氧化程度增加，其与胶原的结合量也增加。结论：这种增加可能主要源于脂蛋白的负电性增加，ＬＤＬ聚集度增大则可降低ＬＤＬ与胶原的结合能力。另外，载脂蛋白Ｂ中赖、精、组、酪氨酸残基的化学修饰。

AIM:To elucidate the relationship between collagen and serum low density lipoprotein (LDL).METHODS:Preparing type Ⅰ collagen gel in vitro and observing the factors which may affect the ability of oxidized low density lipoprotein (ox-LDL) to bind with type Ⅰ collagen.RESULTS: It was found that the amound of LDL bound to collagen increased with the degree of LDL oxidation, which was accompanied by a progressive increase in the negative electric charge and a decrease in the size of lipoprotein. CONCLUSION:Chemically modified lysine, arginine, histidine and tyrosine residues of apolipoprotein B in ox-LDL might play significant role in the binding of ox-LDL with collagen Ⅰ.