再生障碍性贫血与丙型和戊型肝炎病毒相关性的研究

The study on the relativity between hepatitis virus C or E(HCV,HEV) and the occurrance by applying RT/PCR technology to aplastic anemia

目的和方法：采用ＲＴ／ＰＣＲ和定量酶标标准化的测定方法，检测了先患（丙型或戊型）肝炎后患再生障碍性贫血和先患再生障碍性贫血后患丙型（或戊型）肝炎的阳性率，并与对照组比较。结果：血清组中ＨＣＶ－ＲＮＡ的阳性率分别是５７９％和２８９％，二者差异显著（Ｐ＜００５）；ＨＥＶ－ＲＮＡ阳性率分别是２４％和２５９％，二者差异不显著（Ｐ＞００５）。在单个核细胞组中ＨＣＶ－ＲＮＡ阳性率分别为１１８％和１０５％；ＨＥＶ－ＲＮＡ的阳性率分别为０％和３７％；ＨＥＶ－ＲＮＡ在粪便中的阳性率３２％和３７％，均无显著差异。结论：丙型和戊型肝炎病毒不直接影响造血细胞的基因表达，仅使造血微环境有一定改变，引起患者自身免疫反应。

AIM and METHOD: Virus infection may be one of the causes of aplastic anemia(AA).We adopt the RT/PCR technology to examine the positive rate of HCV-RNA(or HEV-RNA)for the purpose to reveal the relativity associated with the occurrance of AA.RESULTS:In two serum groups,the positive rates of HCV-RNA,those followed by AA(called irrelevant AA),those and AA followed by the hepatitis virus C(called relevant AA),are 57.9% and 28.9% respectively,the difference appears significant( P <0 05),the positive rate of HEV-RNA is 24% and 25%,while the difference is not stricking( P >0 05) However,in mononuclear cell,the positive rate of HCV-RNA is 11.8% and 10.5%,HEV-RNA is 0% and 3.7% respectively,meanwhile,in stool,HEV-RNA is 32% and 37%,all are not stricking( P >0 05) CONCLUSION:The occurrance of AA is not related to virus C and E directly,but virus may activate autoimmune response,which makes influence on micro circulation and interferes with hematopoietic function.