红细胞带Ⅲ蛋白C1肽链的纯化及其功能的初步研究

Purification and characterization of C1 peptide released from band 3 protein

目的：研究红细胞带Ⅲ蛋白Ｃ１肽链（带Ⅲ蛋白Ａｌａ８９３－Ｖａ１９１１）的特性及其功能。方法：以１００ｍｍｏｌ／ＬＮａＯＨ预处理红细胞膜血影，再用胰蛋白酶消化，采用高效液相色谱（ＨＰＬＣ）及氨基酸测序仪分析检定了从红细胞血影中释放的多肽，并纯化了带Ⅲ蛋白Ｃ１肽链，以不同浓度的Ｃ１肽链与新鲜的红细胞血影进行温浴。结果：Ｃ１肽链与红细胞内的新蛋白酶活性有关。

AIM: In order to study the properties of C1 peptide(band 3 Ala893-Va1911).METHODS:The human erythrocyte white ghost were pre-treated by 100 mmol/L NaOH and then digested by trypsin. Using the HPLC and amino acid sequencer, we identified the peptides that released from white ghost, and purified the C1 peptides(band 3 Ala893-Va1911). The C1 peptides were used to incubate with fresh white ghosts.RESULTS:C1 peptides related with a novel protease activity.