抗癌药物对乳腺癌细胞动力学及凋亡基因Bc1-2/Bax的影响

Effect of anticancer-drugs on primary breast cancer cell kinetics and apoptesis genes bcl-2/Bax protein expression

探讨抗癌药物对乳腺癌细胞动力学及凋亡基因Ｂｃｌ－２／Ｂａｘ的影响。方法用流式细胞术测定乳腺癌细胞核ＤＮＡ含量（ＤＩ）、Ｓ期细胞比率（ＳＰＦ）、细胞凋亡指数（ＡＩ）、凋亡基因Ｂｃｌ－２、Ｂａｘ的表达及雌激素受体（ＥＲ）等进行定量分析。结果①用药组与对照组相比，ＤＩ、ＳＰＦ及Ｂａｘ明显低于对照组（Ｐ＜０．０１），而ＡＩ（Ｐ＜０．０５）、Ｂｃｌ－２（Ｐ＜０．００１）则高于对照组，ＥＲ于两组间无统计学差异；②ＡＩ与ＳＰＦ、Ｂｃｌ－２及ＥＲ的表达均呈正相关，而与Ｂａｘ无明显相关关系。结论抗癌药物主要通过细胞凋亡而发挥作用，用药后的生物学指标可表现为ＡＩ及Ｂｃｌ－２升高，ＳＰＦ、ＤＩ值及Ｂａｘ下降；ＡＩ与ＳＰＦ、Ｂｃｌ－２及ＥＲ均呈正相关。根据这些指标可判定药物的疗效，并可用于预后的预测。

Objective To study the effect of anticancer-drugs on primary breast cancer cell kinetics andapoptosis genes Bc1-2/Bax protein expression. Method Kinetic targets DNA index (DI) and S-phase fraction(SPF), apoptosis genes Bcl-2/Bax expression, apoptotic index (AI) and estrogen receptor (ER) were examined by using flow cytometry in the primary breast cancer patients with or without pre-chemotherapy. ResultAs compared with control group, a significant decrease in DI (P<0.001), SPE (P<0.001), and Baxprotein expression (P<0.001) was found, but AI (0. 05>P>0.01) and Bcl-2 protein expression (P<0. 001) displayed a significant increase in pre-chemotherapy group. No significant difference in ER was foundbetween the two groups. High percentage of AI was positively correlated with high percentage of SPF, highexpression of Bcl-2 and ER protein. But Bax showed no relation with AI. Conclusion The anticancer-drugsgave their effects by inducing cancer cell apoptosis. The above parameters can be used to judge the anticancerdrugs efficiencies and to predict prognosis.