HMBA对粘液表皮样癌P53蛋白表达和细胞增殖周期的影响

Effects of Hexamethylene Bisacetamide on P53 Protein Expression of MEC 1 Cell Line and Cell Differentiation

目的：研究六亚甲基二乙酰胺（ＨＭＢＡ）诱导人粘液表皮样癌（ＭＥＣ－１）细胞分化与细胞Ｐ５３蛋白表达和细胞增殖周期的关系。方法：ＭＴＴ比色法测定ＨＭＢＡ对ＭＥＣ－１细胞体外生长的作用；Ｆｅｕｌｇｅｎ染色测定细胞ＤＮＡ含量；ＡＢＣ免疫酶染色检测Ｐ５３蛋白；ＦＣＭ法测定细胞增殖周期。结果：ＨＭＢＡ对ＭＥＣ－１细胞的生长抑制作用呈剂量依赖性和时间依赖性；ＨＭＢＡ诱导的细胞与对照细胞比较，ＤＮＡ含量减少，Ｐ５３蛋白水平降低，Ｇ１期细胞数增加和Ｓ期细胞数减少。结论：ＨＭＢＡ对ＭＥＣ－１细胞的诱导分化作用与其调节Ｐ５３基因蛋白表达和细胞增殖周期有关。

Objective:To investigate the relationship between the differentiation of the mucoepidermoid carcinoma cell line MEC 1 induced by HMBA and the expression of P53 protein.Methods: Effects of HMBA on the growth of MEC 1 cells in vitro were measured with MTT colorimetric assay, and the content of DNA in cells was assayed by using Feulgen's staining. The P53 protein expression of MEC 1 cells was detected with ABC immunohistochemistry with anti mutant P53 protein antibody, and the distribution of cells in different differentiation phase was determined by flow cytometry.Results: HMBA inhibited the growth of MEC 1 cells in a dose dependant and time dependant manner, and HMBA treated MEC 1 cells were arrested in the G1 phase and had a lower content of DNA and a lower level of P53 protein compared with the control group.Conclusion:The differentiation of MEC 1 cells induced by HMBA may be associated with the regulation of HMBA on the P53 protein expression and the cell differentiation cycle.