生物反应器中脱色菌Kingella H固定化特性的研究

Study on Immobilization Characters of Decolorizing Bacterium Kingella H in the Bioreactor

对脱色菌KingellaH在活性炭载体表面的固定化特性进行了研究。考察了初始菌悬液浓度、液相离子强度、温度和pH值对微生物在载体上附着固定量的影响。结果表明,随着初始菌悬液浓度的增加,载体上的固定生物量也逐渐增大并趋于稳定;固定生物量与液相中的离子强度成正比,特别是在NaCl为0.01～0.20 mol·L^-1的范围内增加显著;当初始菌悬液浓度为111.1 mg·L^-1、液相离子强度为0.20 mol·L^-1、温度为35℃、pH值为6时,固定生物量最大,达到30μg·cm^-2。动力学研究表明,KingellaH在活性炭表面的附着固定行为遵循一级可逆反应动力学,其附着固定动力学方程式为：1/B=1.46971/t＋0.025。

The immobilization characters of decolorizing bacterium Kingella H on the activated carbon were studied.The influences of initial suspending bacterium concentration,liquid ionic strength,temperature and pH value on the fixed biomass were investigated.The results indicated that the initial suspending bacterium concentration was higher,the fixed biomass on the carrier was more and gradually tended to a stable value.The fixed biomass was in direct proportion to the ionic strength.When the initial suspending bacterium concentration was 111.1 mg·L^-1,the ionic strength was 0.20 mol·L^-1,the temperature was 35℃ and the pH value was 6,the fixed biomass was most and up to 30 μg·cm^-2.The research about the kinetics process indicated that the behavior of Kingella H adsorbed on the activated carbon follows the first-order reversible reaction kinetics.The adsorption kinetics equation is：1/B=1.46971/t＋0.025.