bFGF mRNA在慢性低氧性肺动脉高压大鼠肺组织中的表达及分布

Expression and Distribution of bFGF mRNA in Lung Tissue of Rat with Chronic Pulmonary Hypertension

为探讨碱性成纤维细胞生长因子（ｂＦＧＦ）在慢性低氧性肺动脉高压肺血管重建中的作用及机理，通过建立慢性低氧性肺动脉高压大鼠模型，测定其肺血流动力学。并用酶联免疫吸附法（ＥＬＩＳＡ）测定肺组织匀浆ｂＦＧＦ含量，以及用原位杂交观察ｂＦＧＦｍＲＮＡ表达及分布变化。结果：①缺氧组肺组织匀浆ｂＦＧＦ含量为１８．６±２．９７Ｐｇ／ｍｌ，明显高于对照组（Ｐ＜０．００１）；②缺氧组肺小动脉ｂＦＧＦｍＲＮＡ表达明显增强。结果显示ｂＦＧＦ参与了慢性低氧性肺动脉高压肺小动脉重建的调控。

To evaluate the role of basic fibroblast growth factor (bFGF) in the development of hypoxic pulmonary hypertension, 30 wistarmale rats were divided into hypoxic group and control group. Rat models with chronic hypoxia induced pulmonary hypertension were established. The pulmonary hemodynamics was measured and the concentration of bFGF in rat lung tissue was determined quantitatively by enzyme linked immunosorbent assay (ELISA). Expression and distribution of bFGF messenger ribonucleic acid (mRNA) in rat lung was observed by in situ hybridization using digoxigenin labeled oligonucleotide probe. The result showed the mean pulmonary artery pressure and the concentration of bFGF in rat lung tissue were 3.96±0 47kPa, 18 06±2 97pg/ml in rats exposed to hypoxia for 3 weeks, respectively; both were significantly higher than those in control group ( P <0 01, P <0 001 respectively); expression of bFGF mRNA in the wall of pulmonary arterioles in rats exposed to hypoxia was stronger than that of control group. bFGF mRNA increased obviously in endothial cells, smooth muscle and advential tissue of pulmonary arterioles. These suggest that bFGF takes part in reconstruction of pulmonary arterioles wall in chronic hypoxic pulmonary hypertension, and the main mechanism of bFGF in modulating the structural remodeling of pulmonary arterioles may be autocrine and paracrine.