HPLC法测定清喉利咽颗粒中柚皮苷、橙皮苷、黄芩苷的含量

HPLC Determination of Naringin,Hesperidin,Baicalin in Qinghouliyan Granules

目的:建立HPLC法测定清喉利咽颗粒中柚皮苷、橙皮苷、黄芩苷含量的方法。方法:采用高效液相色谱法,色谱柱:PhenomenexC18柱(100mm×4.6mm,2.6μm),流动相:以甲醇—0.5%磷酸,流速1.0mL.min-1,检测波长λ=280nm,柱温:30℃。结果:柚皮苷在33.6～840ng/μL范围内线性关系良好(r=0.9997),平均回收率为99.25%,RSD=0.30%(n=6);橙皮苷在19～476ng/μL范围内线性关系良好(r=0.9995),平均回收率为99.21%,RSD=0.50%(n=6);黄芩苷在24～600ng/μL范围内线性关系良好(r=0.9999),平均回收率为99.44%,RSD=0.39%(n=6)。阴性对照无干扰。结论:该法操作简便,结果准确可靠,可作为清喉利咽颗粒的质量控制方法。

Objective ：To establish the HPLC method for determination of Naringin Hesperidin and Baicalin in Qinghouliyan Granules. Methods：A Phenomenex C18 Column （100mm×4.6mm,2.6μm） was used.The mobile phase was methanol-0.5%Phosphoric Acid.The column temperature was 30℃.The waveleng detection were at 280nm,flow rate was 1.0 mL.min^-1.Results：The linear range of Naringin was within 33.6-840ng（r=0.9997）, the average recovery was 99.25%,RSD=0.30%（n=6）;The linear range of Hesperidin was within 19- 476ng （r=0.9995） ,the average recovery was 99.21%,RSD=0.50%（n=6） ;The linear range of Baicalin was within 24-600ng （r=0.9999）, the average revovery was 99.44%,RSD was 0.39%（n=6）.The negative sample had no interference.Conclusion：This method is simple, accurate and suitable for its assaying.h can be used for quality control in production of Qinghouliyan Granules.