大鼠下颌髁突软骨细胞体外培养研究

The Behaviour of Rat Mandibular Condylar Cartilage in Cell Culture

下颌髁突软骨的生长改建一直是人们关注的焦点。本实验选用ＳＤ大鼠下颌髁突软骨，分离培养髁突软骨细胞，用倒置显微镜、扫描电镜、酶组织化学、免疫组织化学等方法研究了培养髁突软骨细胞的生物学特征。结果：培养的髁突软骨细胞呈多角形或梭形，细胞内有大量分泌颗粒，并有重叠生长和集落生长的特性，它们能合成特异的蛋白多糖、碱性磷酸酶、Ⅱ型胶原等。本研究所介绍的大鼠下颌髁突软骨细胞的分离培养技术及鉴定方法。

This study aimed to identify the behaviour of mandbular condylar chondrocytes in vitro. Cells were harvested from the mandibular condyles of 3 week old S.D.rats. Cell structure, morphology and characteristics were assessed by phase contrast microscopy, scanning electron microscopy, enzymohistochemistry and immunohistochemistry. The cultured condylar chondrocytes, stellated or spindle shaped, could grow in several layers and form many cell colonies. They could secret proteoglycans, alkaline phosphatase, type Ⅱ collagen, et al. The methods of isolation, culture and identification of condylar chondrocytes were presented and discussed, which can be adopted in probing further into the cellular mechanism of functional orthopedics.