大肠杆菌HX88108膜孔蛋白初步研究

A Study on Porins of Escherichia coli HX88108

为了解大肠杆菌ＨＸ８８１０８耐药机理中有无膜孔蛋白缺失参与，用十二烷基磺酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）检测了大肠杆菌ＨＸ８８１０８的膜孔蛋白，并与膜孔蛋白标准株进行比较分析。结果发现：大肠杆菌ＨＸ８８１０８具有膜孔蛋白ＯｍｐＦ、ＯｍｐＣ。

To investigate the relationship between porins deficiency and the resistance of Escherichia coli HX88108. The porins of Escherichia coli HX88108 were studied by SDS PAGE and were compared with the porins of standard strains. The result showed that E. coli HX88108 did not lose OmpF and OmpC, and the molecular weights of these two porins were 38kd and 37kd respectively. There is no relationship between the resistance of Escherichia coli HX88108 and porins deficiency.