基于全内反射荧光显微镜的单个心肌细胞内钙信号斑图的介观表征

Mesoscopic Representation on Patterns of Ca^(2+) Signals in Single Heart Cell Based on Total Internal Reflection Fluorescence Microscope

利用基于近场光学原理组建的全内反射荧光显微镜研究单个大鼠心肌细胞内钙离子信号的斑图.结果表明:存在于盖玻片表面约100nm区域的隐失场可较好地照射活体细胞,并能清晰地显示胞内信号;心肌细胞内钙信号包括钙火花、钙靶波、钙平面波和钙螺旋波等形式,钙信号各形式间存在相互作用;在钙诱导钙释放机制作用下,心肌细胞具有较强的可激发特性.

The signals of calcium ions in single rat heart cell were studied by means of a total internal reflection fluorescence microscope made by the theory of near-field optics.The results show that Ca^2 ＋ signals can be detected clearly under the illumination of evanescence which only penetrated cell about 100 nm.Ca^2 ＋ signals in single heart cell show Ca^2 ＋ sparks,Ca^2 ＋ target waves,Ca^2 ＋ plane waves and Ca^2 ＋ spiral waves,and these forms interact to each other.Further more,single heart cells show strong excitability under calcium-induced calcium release mechanism.