摘要
采用试剂盒QIAamp DNA Mini Kit从距今4000年前新疆小河墓地出土的黄牛毛皮中提取出古DNA,并对黄牛线粒体D-loop区部分片段进行了PCR扩增和序列测定.结果表明:所提取的古DNA真实可信;且该方法简便、高效,适用于古代动物毛皮DNA的提取.
DNA was successfully extracted from dried cattle skin which was recovered from Xiaohe cemetery,Xinjiang and dated back to 4 000 years ago via QIAamp DNA Mini Kit.PCR amplification and sequence of mitochondrial D-loop fragment were carried out to evaluate the effect of this method.The results show that the extracted DNA was authentic and reliable.Therefore,this method is simple and efficient to use and is suitable for DNA extraction from ancient dried animal skin.
出处
《吉林大学学报（理学版）》
CAS
CSCD
北大核心
2010年第4期689-693,共5页
Journal of Jilin University:Science Edition
基金
国家自然科学基金(批准号:J0930002)
教育部人文社会科学重点研究基地重大项目(批准号:2009JJD780003)
教育部人文社会科学研究项目(批准号:09YJC780003)
中国博士后科学基金(批准号:20080430156)
中国博士后科学基金特别项目(批准号:200902491)
