吸烟者血清弹性蛋白酶、α_1-抗胰蛋白酶、前胶原Ⅲ肽及丙二醛的检测

Changes of serum elastase,α 1 antitrypsin, procollagen Ⅲ peptide and malonaldehyde in smokers with and without chronic obstructive pulmonary diseases

目的探讨慢性阻塞性肺疾病（ＣＯＰＤ）的高危因素。方法采用病例对照设计方法，对１５４例吸烟伴和不伴ＣＯＰＤ者血清α１抗胰蛋白酶（α１ＡＴ）、前胶原三肽（ＰⅢＰ）、弹性蛋白酶及丙二醛进行检测。结果吸烟可引起血清ＰⅢＰ明显增加；吸烟伴ＣＯＰＤ者血清α１ＡＴ为（１６１３±６４３）μｍｏｌ·ｍｉｎ１·ｍｌ１，明显低于无ＣＯＰＤ者［（２２３０±７８４）μｍｏｌ·ｍｉｎ１·ｍｌ１；Ｐ＜０．０１］。结论提示α１ＡＴ降低在ＣＯＰＤ发病中可能是一重要宿主高危因素，对发现ＣＯＰＤ吸烟易感人群可能会有一定意义。

Objective Serum elastase , α 1 antitrypsin (α 1 AT),procollagen Ⅲ peptide (PⅢP) and malonaldehyde (MDA) were studied in smokers with and without chronic obstructive pulmonary disease (COPD) to explore the roles of these factors in COPD induced by smoking. Methods A design of 154 pair case control study was used. α 1 AT, MDA and PⅢP were determined with colorimetric method, fluo spectrophotometric method and radio immunological assay, respectively, in non smoker control and smokers with and without COPD . Results Serum PⅢP content was increased in smokers and α 1 AT decreased significantly in patients with COPD. Conclusion Decreased α 1 AT may be a susceptible factor in the development of COPD caused by smoking.