清火败毒饮对巨噬细胞高迁移率族蛋白1表达的影响

Effect of qinghuobaiduyin on the expression of high mobility group box chromosomal protein 1 in macrophage

目的:观察中药清火败毒饮方(qinghuobaiduyin,QHBDY)对晚期炎症因子高迁移率族蛋白1(high mobility group box chromosomal protein 1,HMGB1)在RAW264.7巨噬细胞中表达的影响,以了解其抗炎症反应的细胞内源性保护机制。方法:以RAW264.7巨噬细胞为研究对象,通过正常SD大鼠血清、烧伤SD大鼠血清和中药QHBDY治疗后SD大鼠血清干预细胞,应用RT-PCR技术检测细胞中HMGB1表达。结果:正常SD大鼠血清干预RAW264.7巨噬细胞后,HMGB1mRNA的表达量与正常RAW264.7巨噬细胞中HMGB1表达量差异无统计学意义(P>0.05);应用烧伤SD大鼠血清干预RAW264.7巨噬细胞,HMGB1的表达量在干预3 h增高后又降为低水平表达,至18 h后急剧增加,于36 h达到高峰,至48 h仍维持高水平表达;应用中药QHBDY治疗后SD大鼠血清干预RAW264.7巨噬细胞,HMGB1的表达量在干预3 h增高后又降为低水平表达,于24 h出现缓慢增加,但较烧伤SD大鼠血清干预组低,两组差别具有统计学意义(P<0.05)。结论:烧伤血清能引起HMGB1在RAW264.7中的高表达;中药QHBDY治疗后SD大鼠血清能降低RAW264.7中HMGB1的表达。

Objective To observe the expression of high mobility group box chromosomal protein 1（HMGB1） in RAW264.7 macrophages after interfering with burning serum and qinghuobaidu-yin（QHBDY）,and to find out the endogenous protection mechanism of QHBDY resisting inflammation reaction.Methods RT-PCR was used to detect the expression of HMGB1 in RAW264.7 macrophages after interfering RAW264.7 macrophages with normal SD rat serum,burning SD rat serum,and QHBDY feeding SD rat serum.Results Small quantity of HMGB1 mRNA was expressed in RAW264.7.The expression of HMGB1 mRNA fluctuated around the standard level after interfering with normal serum of SD rats.The expression of HMGB1 mRNA rose at 3 h,and then decreased to the standard level;at 18 h,it rose rapidly;at 36 h,it reached the peak;and at 48 h,it remained at the high level after interfering with burning serum.The expression of HMGB1 mRNA increased at 3 h,and then decreased to the standard level.At 24 h,it started to rise after interfering with herb serum,and was lower than that of the burning serum group（P0.05）.Conclusion Burning serum can increase the expression of HMGB1 mRNA in RAW264.7.QHBDY can decrease the high expression of HMGB1 mRNA in RAW264.7 caused by burning serum.