摘要
目的探讨中期因子(midkine,MK)、Ki-67、CD34在正常子宫内膜组织、子宫内膜上皮内瘤变(EIN)组织、子宫内膜癌组织中的表达及其在生物学行为方面的意义。方法 15例正常子宫内膜组织、20例EIN组织及30例子宫内膜癌组织标本,应用免疫组织化学方法(SP法)分别检测MK、Ki-67、CD34的表达情况,并进行统计学分析。结果 MK、Ki-67、CD34在正常子宫内膜、EIN和子宫内膜癌组织中的表达逐渐升高,其在3种组织中表达之间的差异具有统计学意义(P<0.05);MK、Ki-67和CD34的表达与子宫内膜癌的临床分期、组织学分级、淋巴结转移、肿瘤肌层浸润程度等有关(P<0.05),而与宫内膜癌患者的年龄无关(P>0.05);MK与Ki-67在子宫内膜癌中的表达呈正相关(r=0.781,P<0.05)。结论 MK的阳性表达与子宫内膜癌的发生、发展和预后有关,可联合Ki-67和CD34作为一组有价值的肿瘤标记和预后指标。
Objective To investigate the expressions and significance of biological behavior of midkine,Ki-67 and CD34 in normal endometrial tissues,endometrial intraepithelial neoplasia（EIN） and endometrial carcinoma.Methods A total of 15 samples of normal endometrial tissue,20 samples of endometrial intraepithelial neoplasia and 30 samples of endometrial carcinoma were examined for the expression of MK,Ki-67 and CD34 by immunohistochemical method（SP） and statistical analysis of the results.Results The positive expressions of MK,Ki-67 and CD34 in endometrial carcinoma were significantly higher than in endometrial intraepithelial neoplasia and normal endometrial tissue（P0.05）.The expression of MK,Ki-67 and CD34 was associated significantly with a clinical staging,grading,lymphatic metastasis and the degree of myometrial infiltration of the tumor（P0.05）,while it was not associated with age of the patients（P0.05）.The expression of MK was positively correlated with that of Ki-67 and CD34 in endometrial carcinoma（r=0.781,P0.05）.Conclusion The positive expressions of MK are significantly correlated with the pathogenesis,progression and prognosis of endometrial carcinoma.MK in combination with Ki-67 and CD34 may serve as a set of valuable markers and prognostic indicators of endometrial carcinoma.
出处
《徐州医学院学报》
CAS
2010年第7期461-465,共5页
Acta Academiae Medicinae Xuzhou
