羊口疮病毒PCR检测方法的建立与应用

Establishment and Application of PCR Method for Detecting ORFV

根据羊口疮病毒(ORFV)H2L基因序列,设计合成1对引物,通过确定最佳的PCR反应条件,建立了检测羊口疮病毒DNA的PCR检测方法。结果显示,用这1对引物均能扩增出羊口疮病毒507 bp的特异性片段,同时检测口蹄疫病毒和山羊痘病毒,结果均为阴性,最小检出量为5 pg。该方法具有良好的特异性和敏感性,可对临床组织病料中的ORFV进行快速检测。

A pair of specific primers were designed and synthesized according to H2L gene sequence of orf virus（ORFV） and the PCR method for detecting DNA of ORFV was established by determining the optimum reaction condition of PCR.The results showed that the specificity fragment of ORFV 507bp could be amplified by a pair of specific primers.The established PCR method could detect viruses of goat poxvirus or foot-and-mouth disease at the same time and the minimum detectable amount was 5pg.The established PCR method with good specificity and sensitivity can be applied in quick detection of ORFV.