二氧化碳气腹对妊娠中期大鼠妊娠特异性β_1糖蛋白(SP_1)近、远期影响的相关性研究

Short-term and long-term influence of carbon dioxide pneumoperitoneum on the level of pregnancy specific β_1 glycoprotein (SP_1) in second trimester rats

目的探讨不同压力、不同持续时间的CO2气腹对妊娠中期大鼠近、远期血浆、胎盘组织以及羊水中SP1的影响。方法建立妊娠中期CO2气腹的大鼠模型,取不同压力、不同气腹时间各处理组以及对照组一半大鼠的血液、羊水及胎盘组织行固相夹心法酶联免疫吸附实验(ELISA),检测SP1含量变化。余下一半大鼠养至妊娠晚期,同法处理。结果妊娠中期大鼠CO2气腹后近期血浆中SP1变化差异无统计学意义(P>0.05),远期血浆中SP1变化差异有统计学意义(P<0.05)。在相同的气腹压力下,血浆SP1随气腹持续时间延长而降低(P<0.05);在相同的作用时间下,血浆SP1随气腹压力增大而降低(P<0.05)。在胎盘组织及羊水中SP1变化差异无统计学意义(P>0.05)。结论妊娠中期大鼠高气腹压力、长时间CO2气腹后能造成远期血浆SP1的减少,而近期血浆以及近、远期胎盘、羊水中SP1量未见明显降低,血浆SP1的改变先于胎盘组织、羊水出现,可预测胎盘功能不良。

Objective To study the short-term and long-term influence of carbon dioxide pneumoperitoneum on the level of pregnancy specific β1 glycoprotein（SP1）in blood plasma,placenta and amniotic fluid in second trimester rats under different pressure with various duration.Methods A second trimester rat models were set up for carbon dioxide pneumoperitoneum experiment.The level of SP1 in blood plasma,placenta and amniotic fluid were examined in second and late trimester rat models with ELISA from different treatment groups under different pressure and various pneumoperitoneum duration and also in the control groups,and then the change of SP1 content was measured.Results High pressure of carbon dioxide pneumoperitoneum in a long time could lead to reducing SP1 in long-term blood plasma,while those in placenta or amniotic fluid showed no obvious decline.The change of SP1in blood plasma appears before placenta,which could be made good use in forecast of placental dysfunction.Conclusion SP1content of short-term long-term blood plasma and long-term placenta homogenate under the same pneumoperitoneum pressure increase with the increasing of pneumoperitoneum time,irrelevant to pneumoper-itoneum pressure.Carbon dioxide pneumoperitoneum has no impact on short-term placenta homogenate and short-term long-term amniotic fluid.