中药天年饮对衰老大鼠肾功能的保护作用

PROTECTIVE EFFECTS OF TRADITIONAL CHINESE MEDICINE TIANNIANYIN ON RENAL FUNCTION OF AGEING RATS

目的:观察中药天年饮对衰老大鼠肾功能的保护作用。方法:40只SD大鼠随机分为4组,正常组、衰老模型组、天年饮用药组和阴性对照组,每组10只。D-半乳糖连续腹腔注射(200mg/kg/d,连续40d)制作亚急性衰老的大鼠模型,并给予天年饮灌胃(30d)。分别采用黄嘌呤氧化酶法和硫代巴比妥酸法检测大鼠血清SOD活性和MDA含量,谷氨酸脱氢酶两点法检测大鼠血清尿素氮含量、酶法检测血清肌酐含量。结果:天年饮可明显提高衰老大鼠血清SOD活性、降低MDA含量,天年饮用药组与衰老模型组比较差异显著(P<0.05,P<0.01);天年饮可明显降低衰老大鼠血清尿素氮及肌酐含量,天年饮用药组与衰老模型组比较差异显著(P<0.05,P<0.01)。结论:天年饮可提高衰老机体的抗氧化能力,保护肾功能,具有一定延缓衰老的作用。

Objective：To observe the protective effects of traditional Chinese medicine Tiannianyin on renal function of ageing rats.Methods：40 male SD rats were randomly divided into 4 groups and each group had 10 rats：normal gorup,ageing model group,Tiannianyin group and negative control group.The subacutely aging model rats were made by injecting D-gal into abdominal cavity continually（200mg/kg/d,40d） and Tiannianyin was lavaged（30d）.The SOD activity and content of MDA,urea nitrogen and creatinine in serum of rats were respectively detected.Results：Tiannianyin could increase SOD activity and decrease MDA content in serum of ageing model rats obviously;there had obvious differences between Tiannianyin group and ageing model group（P0.05,P0.01）.Tiannianyin could decrease urea nitrogen and creatinine content in serum of ageing model rats significantly;there had significant differences between Tiannianyin group and ageing model group（P0.05,P0.01）.Conclusions：Tiannnianyin can increase the anti-oxidative activity and protect renal function,so has fairly anti-aging effects.