免疫抑制小鼠侵袭性肺烟曲霉菌感染模型建立的研究

Establishment of animal model of invasive pulmonary aspergillosis in immunosuppressed mice

目的:建立免疫抑制小鼠侵袭性烟曲霉菌感染模型,为感染性疾病的研究和治疗提供科学依据。方法:给予昆明种健康小鼠腹腔内一次性注射环磷酰胺(CY)200mg/kg,观察其对小鼠一般状况和白细胞数的影响。4d后,经小鼠双侧鼻孔滴注烟曲霉菌孢子悬液30μl引起侵袭性肺烟曲霉菌病(IPA),并于不同时间点处死小鼠,进行组织培养及病理分析。结果:环磷酰胺注射后,小鼠白细胞数量明显降低,病理切片可见免疫抑制小鼠感染烟曲霉菌后肺组织大量烟曲霉菌聚集,组织坏死,形成肺脓肿。结论:成功建立了免疫抑制小鼠侵袭性肺烟曲霉菌病动物模型,为研究人类烟曲霉菌病的致病机制、诊断和防治奠定了实验基础。

Objective：To establish an experimental animal model of invasive pulmonary aspergillosis in immunosuppressed mice for providing scientific evidence with the research and treatment of such infectious disease. Methods：The healthy mice of Kunming species were given intraperitoneal injection of cyclophosphamide（CY） at single dose of 200 mg/kg,followed by observing the effects on general state and leukocyte count of mice. After 4 days,the aspergillus fumigatus conidia suspension（30 μl/mouse） were dropped into the nares of each mouse to cause invasive pulmonary aspergillosis（IPA）,and the mice were killed at different time point for tissue culturing and pathologic examination. Results：After injection of CY,the leukocyte counts were reduced greatly and the pathological findings revealed massive concentration of aspergillus fumigatus in the lung tissues of the immunosuppressed mice after the infection,with necrotic tissues and formation of pulmonary abscess. Conclusion：An experimental animal model of invasive pulmonary aspergillosis was successfully established,which lays a foundation for the study of aspergillosis pathogenesis,diagnosis and prevention.