期刊文献+

人白细胞介素18cDNA的克隆及序列测定

Cloning and Sequencing of Human Interleukin 18cDNA
全文增补中
导出
摘要 目的获取人IL-18cDNA克隆。方法用RT-PCR方法从人外周血单个核细胞中获取人IL-18cDNA,将其克隆至天然蛋白表达载体PBV220中,并进一步亚克隆至融合蛋白表达载体PGEX-4T-3中,对其进行酶切分析及序列测定。结果测序结果表明克隆至PBV220中的IL-18cDNA包含成熟IL-18蛋白的全部编码序列,酶切分析表明一约470bp的基因片段克隆至PGEX-4T-3中,与理论预计的一致。结论获得了编码人IL-18成熟活性蛋白的cDNA克隆。 Objective To obtain the gene encoding human IL 18 mature active protein.Methods The human IL 18cDNA obtained by RT PCR from PBMC was cloned into PBV220 and subcloned into PGEX 4T 3.The recombinant plasmid was characterized with sequencing and restriction enzyme digestion.Results The result of PBV220 IL 18cDNA sequencing showed that the sequence accorded with that published completely, and restriction enzyme degestion suggested that a fragment of about 470 bp was subcloned into PGEX 4T 3.Conclusion The gene encoding full human IL 18 active protein was obtained.
出处 《上海铁道大学学报》 CAS 1999年第3期5-8,共4页
基金 铁道部科技基金
关键词 白细胞介素18 基因克隆 序列测定 CDNA IL 18 gene clone sequencing human
  • 相关文献

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部