混合菌丝球苯胺净化效能研究

The aniline purifying efficiency of a combined mycelial pellet

为提高苯胺的净化效率,避免菌体流失,维持苯胺降解菌在系统中的浓度,对菌丝球作为生物质载体与工程菌结合形成混合菌丝球的净化效能进行了试验.研究了霉菌Y3与细菌JH-9形成混合菌丝球对苯胺的生物降解效果,将混合菌丝球与细菌JH-9的苯胺净化效能进行了对比,探讨了霉菌Y3与细菌JH-9的接种比例、进水苯胺浓度对混合菌丝球净化效能的影响,并进行了间歇流小试.结果显示,2种菌的最佳接种比例为1 mL霉菌Y3孢子悬液(A620=0.25)与3 mL细菌JH-9悬液(母液细菌浓度1.2×109cfu/mL),苯胺浓度在500 mg/L内均不会对混合菌丝球造成抑制作用.间歇流反应器出水稳定,苯胺去除率达100%,去除率60%左右.

The goals of this research were twofold;to avoid loss of bacterial agents,maintaining the concentration of bacteria degrading aniline in a system,and to improve efficiency when purifying aniline.Tests were done to determine the purifying efficiency of mycelial pellets working as a biomass carrier stabilizing engineered bacteria.The effects of aniline biodegradation were studied for mycelial pellets made from a combination of the mold Y3 and the bacteria JH-9.Purifying efficiencies were compared between the combined mycelial pellet and the bacteria JH-9.The purifying efficiency of the combined mycelial pellet was influenced by its inoculation ratio and the influent concentration of aniline.This was tested in a batch reactor.The results showed that the best inoculation ratio was 1mL fungal spore suspension of Y3（A620=0.25） with 3 mL bacterial suspension of JH-9（bacteria concentration： 1.2×10^9 cfu/mL）.Aniline concentrations of less than 500 mg/L did not cause inhibition for the combined mycelial pellet.The effluent of the batch reactor was stable.Aniline was completely eliminated and the chemical oxygen demand（COD） was reduced 60%.