摘要
目的通过观察高眼压后Müler细胞胶质纤维酸性蛋白(GFAP)的表达情况,探讨Müler细胞在高眼压性视网膜损伤中的作用及意义。方法前房加压灌注法制作大鼠急性高眼压模型,免疫组织化学方法显示视网膜上GFAP的表达,并采用计算机图像分析系统加以定量。结果急性高眼压后视网膜上GFAP阳性染色迅速增加,一段时间后渐消退。结论高眼压状态可诱导Müler细胞发生反应性胶质化,提示Müler细胞可能在高眼压损伤过程中发挥保护作用。
ObjectiveTo investigate the role and significance of the Müller cell in glaucomatous damage by observing expression of glial fibrillary acidic protein (GFAP) in Müller cells under acute ocular hypertension.MethodsThe animal model of acute ocular hypertension was made by perfusing physiological saline into the anterior chamber of rats.The GFAP expression in retina was shown with immunohistochemistry,and was quantitated by computerized morphometry.ResultsGFAP expression increased rapidly following acute ocular hypertension,and decreased gradually after a period of time.ConclusionAcute ocular hypertension can induce Müller cells to undergo reactive gliosis.It suggests that the Müller cell may play an important role in protecting the retina from ocular hypertension.
出处
《眼科研究》
CSCD
1999年第2期104-106,共3页
Chinese Ophthalmic Research