肝细胞癌患者和献血员中庚型肝炎病毒感染检测分析

An analysis of HGV infection ststus among HCC patinetS and donors

目的：调查庚型肝炎病毒（HGV）在肝细胞癌（HCC）患者中的感染情况。方法：采用蛋白酶K裂解法提取血清中HGVRNA，逆转录为cDNA后进行巢式聚合酶链反应，获得238bp的特异性片段者为阳性结果。用此技术对145例HCC患者、69例献血员和304例体检者（正常人群）血清进行了检测分析。结果：145例HCC患者中有21例HGVRNA阳性（14．5％），均为男性患者。献血员69例有2例HGVRNA阳性（2．9％），正常人群304例有2例HGVRNA阳性（0.7％）。结论：HCC患者中HGV感染率明显高于献血员和正常人群，提示HGV与HCC发生相关。

Purpose: TO investigate the hepatitis G vims (HGV) infection status among hepatocellular carcinoma (HCC) patients donors and normal Persons. Methods: Proteinase K digestion method was used to extract HGV RNA in sera.RT－nested PCR technique was applied to amplify the 238 bp HGV genome fragment. Serum specimens of 145 cases of HCC,69 donors and 304 normal persons have been tested. Results: HGV RNA was positive in (1 ) 21 (14. 5% ) male patients out of 145 cases of HCC, (2) 2 (2. 9% ) out of 304 normal individuals, and (3) 2(0. 7% ) out of 69 normal blood donors. Conclusion: Positive rate of HGV RNA in HCC patients was significant higher than that in donors and normal persons, and suggested that HGV infection might be related with HCC.