Cadherin-Catenin复合体及p53蛋白表达与早期胃癌淋巴结转移的关系

THE RELATIONSHIP BETWEEN LYMPH NODE METASTASIS IN EARLY GASTRIC CANCER AND CADHERIN-CATENIN COMPLEX, P53 EXPRESSION

运用单克隆抗Ｅ－ｃａｄ、ａ－ｃａｔ和ｐ５３蛋白抗体及免疫组化方法检测了６９例早期胃癌标本。结果显示，Ｅ－ｃａｄ及ａ－ｃａｔ表达减弱率分别为５３．６％和６５．２％，ｐ５３蛋白阳性率为２７．５％。Ｅ－ｃａｄ及ａ－ｃａｔ的表达显著相关，而ｐ５３的表达与Ｅ－ｃａｄ或α－ｃａｔ表达无关。粘膜下癌α－ｃａｔ减弱表达及ｐ５３蛋白阳性表达者淋巴结转移率较高；而α－ｃａｔ保留表达及ｐ５３蛋白阴性表达者无论是粘膜内或粘膜下癌，未见淋巴结转移。提示结合α－ｃａｔ及ｐ５３蛋白表达，可协助诊断早期胃癌淋巴结转移。

Sixty-nine specimens obtained bysurgery were studied by imunohistochemistry usingmonoclonal anti-E-cadherin, anti-α-catenin and antip53 antibodies. The reduced expression of E-cadherin and a-catenin were 53. 6% and 65. 2% respectively. p53 protein positive staining was 27. 5%.There was significant correlation between E-cad-herin and Q-catenin expression, but no relation wasfound between p53 expression and E-cadherin or a-catenin expression. a-catenin reduced expressionand p53 overexpression correlated to lymph nodemetastasis. Submucosa tumors with positive p53staining and reduced expression of a-catenin weremost likely to have lymph node metastasis, whiletumors with p53 negative and a-catenin presservedexpression had no metastasis in either mucosa orsubmucosa lesions. The results indicate that a combination of a-catenin and p53 expression with pathological features may be helpful to predict the lymphnode metastasis in early gastric cancer.