过氧化作用对慢性阻塞性肺病和肺心病患者淋巴细胞线粒体膜磷脂结构的影响

STUDY OF SUPEROXIDATIVE FUNCTION TO STRUCTURE CHANGE OF MEMBRANE PHOSPHOLIPID OF MITOCHONDRIA IN LYMPHOCYTE OF THE PATIENT WITH COPD AND COR PULMONALE

用放射免疫法和硫代巴比妥酸荧光法分别检测了３６例慢性阻塞性肺病（ＣＯＰＤ）患者和ＣＯＰＤ并肺心病患者、１６例健康人的超氧化物歧化酶（ＳＯＤ）、雨二醛（ＭＤＡ）并同时采用改良的Ｂｏｙｕｎｎ法分离、固定淋巴细胞，对外周血淋巴细胞线粒体膜磷脂进行组织化学定位的研究。结果表明：ＣＯＰＤ和肺心病患者的不同病期与ＳＯＤ、ＭＤＡ、淋巴细胞线粒体数目及膜磷脂的改变有相关性，病程长、病情重，ＭＤＡ则升高，淋巴细胞线粒体数目增多，线粒体膜磷脂定位呈现不同程度的改变。提示：过氧化作用与ＣＯＰＤ和肺心病患者外周血淋巴细胞线粒体膜磷脂的改变有明显的相关性，可能是其影响细胞功能活动的靶部位。

In order to determine the demagingsites of the superoxidative function in cases ofCOPD and Cor pulmonale, we measured the level ofSOD and MDA. We also conducted the histoimmunologic positioning of mitochondrial membranepho%pholipid in the isolated (下转第83页)(上接第77页) lymphocytes in 36 patients withCOPD and Cor pulmonale and 16 health in control.The results showed that the stages of COPD andCor pulmonale correlated with SOD, MDA, thenumber of mitochondria in lymphocytes and thechange of membrane phospholipid significantly. Thelevel of MDA and the number of mitochondric increased in patients with longer history of COPD andCor pulmonale and with more seventy. Our studysuggests that: ① superoxidative function correlateswith the changes of membrane phospholipid significantlyl ② the membrane phospholipid may be thetarget sites of this imbalance, which could affect thefunction of the cells.