“通利大肠”对慢性阻塞性肺疾病模型大鼠氧化应激的影响

Effects of ‘catharsis large intestine’on oxidative stress in rats with COPD

目的:观察"通利大肠"对慢性阻塞性肺疾病(COPD)模型大鼠氧化应激的影响,从氧化/抗氧化失衡角度探讨COPD"从肠论治"的效应机制。方法:采用气管注脂多糖加熏香烟联合造模方法建立COPD大鼠模型,随机分为正常对照组、模型组、治肺组、治肠组及肺肠同治组。正常对照组、模型组灌胃生理盐水,各给药组灌胃相应中药,连续14d。检测血清还原型谷胱甘肽(GSH)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、总抗氧化力(T-AOC)水平。结果:与正常对照组比较,模型组大鼠GSH、SOD和T-AOC降低,MDA升高(P<0.01)。与模型组比较,治肠组MDA降低,GSH、SOD增高(P<0.05)。与治肺组比较,肺肠同治组GSH增高,MDA降低(P<0.01)。结论:通利大肠,或在治肺的基础上增加通利大肠,均能增强对慢性阻塞性肺疾病模型大鼠氧化/抗氧化失衡的改善程度,这可能是COPD"从肠论治"效应产生的肺肠联络机制之一。

Objective： To observe the influence of ‘catharsis large intestine’on oxidative stress in rats with COPD, and to investigate the mechanism of ‘treating from intestine’. Methods： The rat model of COPD was established by cigarette smoking combined with intratracheal injection of LPS. All rats were randomly divided into normal control group, model group, treating lung group, treating intestine group, treating lung and intestine group. The normal control group, model group were intragastrically given the normal saline solution, and other groups were intragastrically given corresponding herbal drugs for 14 days. Using spectrophotometry to detect content of serum GSH, MDA, SOD, T-AOC. Results： The levels of serum GSH, SOD, T-AOC were markedly lower, while MDA was higher, in the model group, compared with those in the normal control group （P〈0.01）. MDA level was lower and GSH, SOD levels were higher in the intestine group than those in model group（P〈0.05）. MDA level was lower and GSH levels was higher in the lung group than those in the lung and intestine group（P〈0.01）. Conclusion： ‘catharsis large intestine’ can enhance the improving in unbalance of oxidation and resistant to oxidation.